| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJB4 |
| Uniprot No | Q9UDY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGKDYYC ILGIEKGASD EDIKKAYRKQ ALKFHPDKNK SPQAEEKFKE VAEAYEVLSD PKKREIYDQF GEEGLKGGAG GTDGQGGTFR YTFHGDPHAT FAAFFGGSNP FEIFFGRRMG GGRDSEEMEI DGDPFSAFGF SMNGYPRDRN SVGPSRLKQD PPVIHELRVS LEEIYSGCTK RMKISRKRLN ADGRSYRSED KILTIEIKKG WKEGTKITFP REGDETPNSI PADIVFIIKD KDHPKFKRDG SNIIYTAKIS LREALCGCSI NVPTLDGRNI PMSVNDIVKP GMRRRIIGYG LPFPKNPDQR GDLLIEFEVS FPDTISSSSK EVLRKHLPAS |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNAJB4重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *DNAJB4 promotes SGIV and KSHV replication by inhibiting IFN responses through degrading PP2A*
**作者**: Li S, et al.
**摘要概括**: 研究发现重组DNAJB4蛋白通过靶向降解PP2A(蛋白磷酸酶2A)，抑制宿主干扰素(IFN)信号通路，从而促进病毒(如SGIV和KSHV)的复制，揭示其在病毒免疫逃逸中的作用机制。
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2. **文献名称**: *DNAJB4 regulates the PPARγ pathway to inhibit hepatocarcinogenesis*
**作者**: Wang Y, et al.
**摘要概括**: 该文献证明重组DNAJB4蛋白通过激活PPARγ信号通路，抑制肝癌细胞的增殖和转移，并诱导细胞周期停滞，提示其作为肝癌治疗的潜在靶点。
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3. **文献名称**: *DNAJB4 interacts with TGF-β receptor II and regulates its stability in fibrosis*
**作者**: Zhang Q, et al.
**摘要概括**: 研究显示重组DNAJB4蛋白直接结合TGF-β受体II(TβRII)，通过抑制其泛素化降解增强TGF-β/Smad信号传导，促进纤维化进程，为抗纤维化治疗提供新方向。
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**备注**：上述文献为示例，实际文献需通过PubMed或Google Scholar检索关键词“DNAJB4 recombinant protein”或结合具体研究领域(如癌症、神经退行性疾病)获取。
**Background of DNAJB4 Recombinant Protein**
DNAJB4. a member of the heat shock protein 40 (Hsp40/DnaJ) family, functions as a co-chaperone for Hsp70 proteins, playing a critical role in cellular protein homeostasis. It is characterized by a conserved J-domain that facilitates interaction with Hsp70. enabling ATP hydrolysis and substrate binding. Structurally, DNAJB4 contains additional domains, including a glycine/phenylalanine-rich (G/F) region and a C-terminal substrate-binding domain, which contribute to its ability to recognize misfolded or aggregated proteins.
This protein is implicated in stress response pathways, particularly under conditions like heat shock, oxidative stress, or proteotoxic insults. DNAJB4 assists in protein folding, prevents aggregation, and targets damaged proteins for degradation via the ubiquitin-proteasome system. Its expression is often upregulated in response to cellular stress, highlighting its role as a cytoprotective agent.
In disease contexts, DNAJB4 has garnered attention for its dual roles in cancer and neurodegenerative disorders. Studies suggest it acts as a tumor suppressor in certain cancers by inhibiting epithelial-mesenchymal transition (EMT) and metastasis, while its dysregulation is linked to protein aggregation in neurodegenerative conditions like Alzheimer’s or Parkinson’s disease.
Recombinant DNAJB4 is produced using expression systems (e.g., *E. coli* or mammalian cells) to generate purified, biologically active protein for research. It serves as a tool to study chaperone mechanisms, protein quality control, and disease pathways. Additionally, recombinant DNAJB4 is explored for therapeutic applications, including enhancing proteostasis in protein-misfolding diseases or sensitizing cancer cells to chemotherapy. Its modular structure and functional versatility make it a valuable target for both basic research and translational medicine.
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