| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKh |
| Uniprot No | Q86XP1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1220aa |
| 氨基酸序列 | MAGAGGQHHPPGAAGGAAAGAGAAVTSAAASAGPGEDSSDSEAEQEGPQKLIRKVSTSGQIRTKTSIKEGQLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHLTKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKDLGIPKVGHVKRILQGIKELGRSTPQSEV |
| 预测分子量 | 134,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGKh(二酰基甘油激酶η)重组蛋白研究的假设性参考文献示例(实际文献需通过数据库核实)：
1. **《重组DGKh蛋白的异源表达与酶活性分析》**
- 作者：Smith A, et al.
- 摘要：研究通过大肠杆菌系统成功表达DGKh重组蛋白，优化纯化条件并验证其催化二酰基甘油(DAG)转化为磷脂酸(PA)的酶活性，为体外功能研究提供基础工具。
2. **《DGKh重组蛋白在T细胞信号转导中的作用机制》**
- 作者：Li X, Zhang Y.
- 摘要：利用杆状病毒-昆虫细胞系统表达DGKh重组蛋白，发现其通过调控DAG/PA平衡影响T细胞受体信号通路，揭示其在免疫调节中的潜在作用。
3. **《DGKh重组蛋白的结构解析及其底物特异性研究》**
- 作者：Wang C, et al.
- 摘要：通过X射线晶体学解析DGKh重组蛋白的催化结构域，结合突变实验阐明其底物结合位点，为开发特异性抑制剂提供结构基础。
4. **《DGKh重组蛋白在代谢疾病模型中的应用》**
- 作者：Kim J, et al.
- 摘要：在肝细胞模型中过表达DGKh重组蛋白，发现其通过降低细胞内DAG水平改善胰岛素抵抗，提示DGKh可能作为代谢疾病的治疗靶点。
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**注**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词如“DGKh recombinant”“Diacylglycerol kinase eta expression”等获取。
DGKh (diacylglycerol kinase η) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DAG) to produce phosphatidic acid (PA), playing a critical role in regulating lipid-mediated signaling pathways. As a member of the diacylglycerol kinase (DGK) family, DGKh belongs to the type II subfamily, characterized by unique structural domains such as a pleckstrin homology (PH) domain and sterile alpha motif (SAM) domains, which facilitate membrane association and protein-protein interactions. It is primarily expressed in the brain, immune cells, and certain cancer tissues, where it modulates cellular processes like proliferation, apoptosis, and cytoskeletal reorganization.
Recombinant DGKh protein is engineered through molecular cloning techniques, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications and functional activity. Its production enables detailed biochemical studies, including kinase activity assays, structural analysis, and interaction mapping with signaling partners like RasGRP1 and mTOR. Researchers utilize DGKh recombinant proteins to investigate its dual role in attenuating DAG-dependent signaling (e.g., PKC/Ras pathways) while promoting PA-mediated cascades (e.g., mTOR/SPHK pathways), which are implicated in diseases such as cancer, neurological disorders, and immune dysregulation.
Recent studies highlight DGKh's potential as a therapeutic target, particularly in cancers with hyperactive lipid signaling. Its recombinant form also aids in drug discovery, serving as a tool for screening inhibitors or modulators to restore dysregulated lipid metabolism in pathological conditions. Ongoing research aims to clarify tissue-specific functions and its interplay with other DGK isoforms, advancing precision medicine approaches targeting lipid kinase networks.
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