| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTL2 |
| Uniprot No | Q8IWA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706aa |
| 氨基酸序列 | MGDERPHYYGKHGTPQKYDPTFKGPIYNRGCTDIICCVFLLLAIVGYVAVGIIAWTHGDPRKVIYPTDSRGEFCGQKGTKNENKPYLFYFNIVKCASPLVLLEFQCPTPQICVEKCPDRYLTYLNARSSRDFEYYKQFCVPGFKNNKGVAEVLQDGDCPAVLIPSKPLARRCFPAIHAYKGVLMVGNETTYEDGHGSRKNITDLVEGAKKANGVLEARQLAMRIFEDYTVSWYWIIIGLVIAMAMSLLFIILLRFLAGIMVWVMIIMVILVLGYGIFHCYMEYSRLRGEAGSDVSLVDLGFQTDFRVYLHLRQTWLAFMIILSILEVIIILLLIFLRKRILIAIALIKEASRAVGYVMCSLLYPLVTFFLLCLCIAYWASTAVFLSTSNEAVYKIFDDSPCPFTAKTCNPETFPSSNESRQCPNARCQFAFYGGESGYHRALLGLQIFNAFMFFWLANFVLALGQVTLAGAFASYYWALRKPDDLPAFPLFSAFGRALRYHTGSLAFGALILAIVQIIRVILEYLDQRLKAAENKFAKCLMTCLKCCFWCLEKFIKFLNRNAYIMIAIYGTNFCTSARNAFFLLMRNIIRVAVLDKVTDFLFLLGKLLIVGSVGILAFFFFTHRIRIVQDTAPPLNYYWVPILTVIVGSYLIAHGFFSVYGMCVDTLFLCFLEDLERNDGSAERPYFMSSTLKKLLNKTNKKAAES |
| 预测分子量 | 80,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTL2重组蛋白的3篇参考文献示例(注：内容为模拟虚构，仅供格式参考)：
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1. **文献名称**: *"Expression and functional characterization of recombinant CTL2 in mammalian cells"*
**作者**: Köhler R, et al.
**摘要**: 本研究成功在HEK293细胞中表达并纯化了CTL2重组蛋白，证实其具有胆碱转运活性，并揭示了其在维持细胞膜脂质代谢中的潜在作用。
2. **文献名称**: *"Structural insights into CTL2-mediated immune regulation"*
**作者**: Iwama T, et al.
**摘要**: 通过冷冻电镜解析了CTL2重组蛋白的三维结构，发现其与MHC-I类分子存在相互作用，提示CTL2可能参与T细胞介导的自身免疫性内耳疾病病理过程。
3. **文献名称**: *"CTL2 as a therapeutic target in autoimmune hearing loss"*
**作者**: Compton A, et al.
**摘要**: 利用CTL2重组蛋白建立动物模型，证明抗CTL2抗体会导致耳蜗毛细胞损伤，为免疫靶向治疗感音神经性耳聋提供了实验依据。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“CTL2 recombinant protein”或“CTL2 choline transporter”获取最新研究。
**Background of CTL2 Recombinant Protein**
CTL2 (Choline Transporter-Like Protein 2), encoded by the *SLC44A2* gene, belongs to the solute carrier 44 (SLC44) family, which mediates choline transport across cell membranes. Choline is essential for synthesizing phosphatidylcholine (a key phospholipid in cell membranes) and acetylcholine (a neurotransmitter). Unlike other choline transporters, CTL2 is widely expressed in non-neuronal tissues, including the inner ear, placenta, and immune cells, suggesting diverse physiological roles.
CTL2 gained attention due to its association with autoimmune inner ear disease (AIED), where it acts as an autoantigen triggering immune responses that damage cochlear cells, leading to hearing loss. This discovery highlighted its potential role in immune regulation and auditory function. Structurally, CTL2 is a transmembrane protein with 10 predicted helices, though its precise transport mechanism remains under investigation.
Recombinant CTL2 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), retains structural and functional properties for *in vitro* studies. It enables researchers to explore CTL2’s interaction with antibodies, choline transport kinetics, and signaling pathways. Additionally, recombinant CTL2 serves as a tool for developing diagnostic assays or therapeutic strategies targeting autoimmune hearing disorders. Recent studies also link *SLC44A2* polymorphisms to thrombosis and cardiovascular diseases, expanding CTL2’s relevance beyond auditory biology.
Overall, CTL2 recombinant protein is a critical reagent for dissecting choline metabolism, immune-mediated pathologies, and cellular homeostasis, offering translational potential in both biomedical research and clinical applications.
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