| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SDK1 |
| Uniprot No | Q7Z5N4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-2213aa |
| 氨基酸序列 | MARGARPSAAGGGGGGAEPPERAGPGRPRGSPPGRARPSLAPRPGPEPSRPRAAPETSGGDTAGAGRCGGRRAAKLGPGRRGWWALLALQLHLLRALAQDDVAPYFKTEPGLPQIHLEGNRLVLTCLAEGSWPLEFKWMRDDSELTTYSSEYKYIIPSLQKLDAGFYRCVVRNRMGALLQRKSEVQVAYMGSFMDTDQRKTVSQGRAAILNLLPITSYPRPQVTWFREGHKIIPSNRIAITLENQLVILATTTSDAGAYYVQAVNEKNGENKTSPFIHLSIARDVGTPETMAPTIVVPPGNRSVVAGSSETTLECIASARPVEDLSVTWKRNGVRITSGLHSFGRRLTISNPTSADTGPYVCEAALPGSAFEPARATAFLFIIEPPYFTAEPESRISAEVEETVDIGCQAMGVPLPTLQWYKDAISISRLQNPRYKVLASGGLRIQKLRPEDSGIFQCFASNEGGEIQTHTYLDVTNIAPVFTQRPVDTTVTDGMTAILRCEVSGAPKPAITWKRENHILASGSVRIPRFMLLESGGLQIAPVFIQDAGNYTCYAANTEGSLNASATLTVWNRTSIVHPPEDHVVIKGTTATLHCGATHDPRVSLRYVWKKDNVALTPSSTSRIVVEKDGSLLISQTWSGDIGDYSCEIVSEGGNDSRMARLEVIELPHSPQNLLVSPNSSHSHAVVLSWVRPFDGNSPILYYIVELSENNSPWKVHLSNVGPEMTGVTVSGLTPARTYQFRVCAVNEVGRGQYSAETSRLMLPEEPPSAPPKNIVASGRTNQSIMVQWQPPPETEHNGVLRGYILRYRLAGLPGEYQQRNITSPEVNYCLVTDLIIWTQYEIQVAAYNGAGLGVFSRAVTEYTLQGVPTAPPQNVQTEAVNSTTIQFLWNPPPQQFINGINQGYKLLAWPADAPEAVTVVTIAPDFHGVHHGHITNLKKFTAYFTSVLCFTTPGDGPPSTPQLVWTQEDKPGAVGHLSFTEILDTSLKVSWQEPLEKNGIITGYQISWEVYGRNDSRLTHTLNSTTHEYKIQGLSSLTTYTIDVAAVTAVGTGLVTSSTISSGVPPDLPGAPSNLVISNISPRSATLQFRPGYDGKTSISRWIVEGQVGAIGDEEEWVTLYEEENEPDAQMLEIPNLTPYTHYRFRMKQVNIVGPSPYSPSSRVIQTLQAPPDVAPTSVTVRTASETSLRLRWVPLPDSQYNGNPESVGYRIKYWRSDLQSSAVAQVVSDRLEREFTIEELEEWMEYELQMQAFNAVGAGPWSEVVRGRTRESVPSAAPENVSAEAVSSTQILLTWTSVPEQDQNGLILGYKILFRAKDLDPEPRSHIVRGNHTQSALLAGLRKFVLYELQVLAFTRIGNGVPSTPLILERTKDDAPGPPVRLVFPEVRLTSVRIVWQPPEEPNGIILGYQIAYRLASSSPHTFTTVEVGATVRQFTATDLAPESAYIFRLSAKTRQGWGEPLEATVITTEKRERPAPPRELLVPQAEVTARSLRLQWVPGSDGASPIRYFTMQVRELPRGEWQTYSSSISHEATACVVDRLRPFTSYKLRLKATNDIGDSDFSSETEAVTTLQDVPGEPPGSVSATPHTTSSVLIQWQPPRDESLNGLLQGYRIYYRELEYEAGSGTEAKTLKNPIALHAELTAQSSFKTVNSSSTSTMCELTHLKKYRRYEVIMTAYNIIGESPASAPVEVFVGEAAPAMAPQNVQVTPLTASQLEVTWDPPPPESQNGNIQGYKIYYWEADSQNETEKMKVLFLPEPVVRLKNLTSHTKYLVSISAFNAAGDGPKSDPQQGRTHQAAPGAPSFLAFSEITSTTLNVSWGEPAAANGILQGYRVVYEPLAPVQGVSKVVTVEVRGNWQRWLKVRDLTKGVTYFFRVQARTITYGPELQANITAGPAEGSPGSPRDVLVTKSASELTLQWTEGHSGDTPTTGYVIEARPSDEGLWDMFVKDIPRSATSYTLSLDKLRQGVTYEFRVVAVNEAGYGEPSNPSTAVSAQVEAPFYEEWWFLLVMALSSLIVILLVVFALVLHGQNKKYKNCSTGKGISTMEESVTLDNGGFAALELSSRHLNVKSTFSKKNGTRSPPRPSPGGLHYSDEDICNKYNGAVLTESVSLKEKSADASESEATDSDYEDALPKHSFVNHYMSDPTYYNSWKRRAQGRAPAPHRYEAVAGSEAGAQLHPVITTQSAGGVYTPAGPGARTPLTGFSSFV |
| 预测分子量 | 242 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SDK1重组蛋白的假设性参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**：*Structural characterization of recombinant SDK1 and its role in synaptic adhesion*
**作者**：Smith J, et al.
**摘要**：通过表达并纯化重组SDK1蛋白，解析其晶体结构，揭示了其免疫球蛋白样结构域在神经元突触黏附中的关键作用，为研究神经发育机制提供了分子基础。
2. **文献名称**：*Recombinant SDK1 enhances axonal regeneration in a rodent nerve injury model*
**作者**：Li X, et al.
**摘要**：研究利用大肠杆菌表达的重组SDK1蛋白，证明其在体外和体内模型中显著促进轴突再生，提示其在神经修复治疗中的潜在应用价值。
3. **文献名称**：*SDK1 recombinant protein interacts with integrin β1 to modulate cancer cell migration*
**作者**：Wang Y, et al.
**摘要**：通过重组SDK1蛋白的体外结合实验，发现其与整合素β1的相互作用可激活下游信号通路，促进乳腺癌细胞的迁移和侵袭。
4. **文献名称**：*Functional analysis of SDK1 isoforms using recombinant proteins in zebrafish development*
**作者**：Garcia R, et al.
**摘要**：利用重组SDK1异构体蛋白研究其在斑马鱼胚胎发育中的功能差异，发现特定异构体对血管生成具有调控作用。
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**注意**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“SDK1 recombinant protein”或“SDK1 structure/function”检索。若需具体文献，建议补充研究领域或应用方向以缩小范围。
SDK1 (SIDEKICK 1) is a cell adhesion molecule belonging to the immunoglobulin (Ig) superfamily, first identified for its role in mediating specific intercellular interactions during tissue development. It shares structural homology with other Ig-like proteins, characterized by extracellular Ig-like domains and fibronectin type III repeats, which facilitate cell-surface recognition and binding. SDK1 is prominently expressed in developing neural tissues and the retina, where it guides synaptic connectivity and cellular patterning by establishing selective adhesive interactions between neighboring cells. Its function is critical in processes like axon guidance, neuronal circuit formation, and retinal layer stratification.
The interest in recombinant SDK1 protein stems from its potential applications in studying neurodevelopmental mechanisms and disease models. Recombinant SDK1 is produced via heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and functional fidelity. This engineered protein enables researchers to probe SDK1’s binding partners, signaling pathways, and role in tissue morphogenesis through in vitro assays or animal studies. Additionally, dysregulation of SDK1 has been implicated in neurological disorders and cancers, making it a candidate for therapeutic targeting. For instance, aberrant SDK1 expression correlates with tumor progression in certain carcinomas, suggesting its involvement in cell migration and metastasis. Recombinant SDK1 tools are also used to develop antibodies or inhibitors that modulate its activity, offering avenues for drug discovery. Overall, SDK1 recombinant protein serves as a vital resource for decoding its biological significance and translating findings into clinical interventions.
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