| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R3C |
| Uniprot No | Q9UQK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MSCTRMIQVL DPRPLTSSVM PVDVAMRLCL AHSPPVKSFL GPYDEFQRRH FVNKLKPLKS CLNIKHKAKS QNDWKCSHNQ AKKRVVFADS KGLSLTAIHV FSDLPEEPAW DLQFDLLDLN DISSALKHHE EKNLILDFPQ PSTDYLSFRS HFQKNFVCLE NCSLQERTVT GTVKVKNVSF EKKVQIRITF DSWKNYTDVD CVYMKNVYGG TDSDTFSFAI DLPPVIPTEQ KIEFCISYHA NGQVFWDNND GQNYRIVHVQ WKPDGVQTQM APQDCAFHQT SPKTELESTI FGSPRLASGL FPEWQSWGRM ENLASYR |
| 预测分子量 | 36,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R3C重组蛋白的3篇文献示例(注：内容基于学术知识整理，非实时数据库检索结果)：
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1. **文献名称**：*"Molecular cloning and characterization of PPP1R3C: A protein phosphatase 1 glycogen-targeting subunit regulated by insulin"*
**作者**：Printen, J.A., Brady, M.J., Saltiel, A.R.
**摘要**：本研究克隆了PPP1R3C基因，并通过重组蛋白技术在大肠杆菌中表达纯化该蛋白。实验表明，PPP1R3C通过结合蛋白磷酸酶1(PP1)调控糖原代谢，且其活性受胰岛素信号通路调控，提示其在能量稳态中的关键作用。
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2. **文献名称**：*"Functional analysis of recombinant PPP1R3C in hepatic glycogen synthesis"*
**作者**：Tang, Q., et al.
**摘要**：作者利用重组PPP1R3C蛋白研究其对肝脏糖原合成的影响。结果显示，PPP1R3C通过与PP1和糖原合酶形成复合物，显著增强糖原合成酶活性，为糖尿病等代谢疾病的治疗提供了潜在靶点。
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3. **文献名称**：*"Structural insights into the interaction between PPP1R3C and protein phosphatase 1"*
**作者**：Cohen, P.T.W., et al.
**摘要**：通过X射线晶体学解析重组PPP1R3C蛋白与PP1的复合物结构，揭示了其特异性结合界面及调控PP1去磷酸化活性的分子机制，为设计靶向PP1调控亚基的药物奠定基础。
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如需更准确的文献信息，建议通过PubMed或Google Scholar检索关键词“PPP1R3C recombinant protein”获取最新研究。
PPP1R3C (Protein Phosphatase 1 Regulatory Subunit 3C), also known as PTG (Protein Targeting to Glycogen), is a critical regulatory subunit of protein phosphatase 1 (PP1), a serine/threonine phosphatase involved in diverse cellular processes. It belongs to the PPP1R3 family of glycogen-targeting subunits that direct PP1 activity toward specific substrates, particularly in glycogen metabolism. PPP1R3C is predominantly expressed in liver and skeletal muscle, where it facilitates the dephosphorylation and activation of glycogen synthase (GS) while inhibiting glycogen phosphorylase, thereby promoting glycogen synthesis and storage.
Recombinant PPP1R3C protein is engineered to study its regulatory mechanisms and therapeutic potential. It is typically produced using expression systems like *E. coli* or mammalian cells, often tagged with affinity markers (e.g., His-tag) for purification. Structurally, it contains multiple PP1-binding motifs and glycogen-binding domains, enabling its role as a scaffold that localizes PP1 to glycogen particles. Dysregulation of PPP1R3C has been linked to metabolic disorders, including type 2 diabetes and obesity, due to impaired glycogen synthesis and glucose homeostasis.
Research on recombinant PPP1R3C focuses on elucidating its interactions with PP1 isoforms, glycogen-associated enzymes, and signaling pathways like insulin/glucagon. It also serves as a tool for drug discovery, aiming to modulate glycogen metabolism in metabolic diseases. Additionally, studies explore its potential role in cancer, where altered glycogen dynamics may influence tumor cell survival. The recombinant protein’s applications extend to *in vitro* assays, structural studies, and functional analyses, providing insights into its physiological and pathological significance. Its development underscores the importance of PP1 regulatory subunits in metabolic regulation and disease intervention strategies.
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