| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP2s1 |
| Uniprot No | P53680 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE |
| 预测分子量 | 23.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP2S1重组蛋白的模拟参考文献示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**: *"Expression and Purification of Recombinant AP2S1 for Structural Studies"*
**作者**: Smith J. et al.
**摘要**: 本研究报道了人源AP2S1重组蛋白在大肠杆菌中的高效表达及纯化方法，通过X射线晶体学解析了其与钙离子的结合结构，揭示了其在AP-2复合体组装中的构象变化机制。
2. **文献名称**: *"Functional Analysis of AP2S1 Mutants in Clathrin-Mediated Endocytosis"*
**作者**: Chen L. et al.
**摘要**: 通过构建AP2S1重组蛋白的R15Q和R15W突变体，发现其与AP2α亚基的结合能力显著降低，证实AP2S1的特定残基突变可破坏网格蛋白包被囊泡的形成，影响细胞内吞功能。
3. **文献名称**: *"AP2S1 Recombinant Protein Interaction with Calcium Signaling Pathways"*
**作者**: Gupta R. et al.
**摘要**: 利用重组AP2S1蛋白进行体外结合实验，证明其通过钙调蛋白依赖性方式参与细胞内钙稳态调控，并揭示了其在非内吞相关信号通路中的潜在作用。
4. **文献名称**: *"Cryo-EM Structure of the AP-2 Complex Bound to AP2S1"*
**作者**: Müller T. et al.
**摘要**: 基于重组AP2S1蛋白与AP-2复合体的冷冻电镜结构解析，阐明了AP2S1在复合体稳定性中的关键作用，并提出了其调控货物蛋白识别的分子模型。
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注：以上文献为模拟内容，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。
**Background of AP2σ1 Recombinant Protein**
Adaptor Protein 2 complex subunit sigma 1 (AP2σ1 or AP2S1) is a critical component of the heterotetrameric AP-2 complex, a central player in clathrin-mediated endocytosis (CME). This complex facilitates the internalization of transmembrane cargo, such as receptors and lipids, by linking clathrin coats to vesicle membranes and recruiting accessory proteins. AP2σ1. encoded by the *AP2S1* gene, is the smallest subunit (σ1) of the AP-2 complex and contributes to its structural stability and functional specificity.
The AP-2 complex consists of α, β2. μ2. and σ1 subunits, with σ1 playing a key role in recognizing tyrosine-based sorting signals (e.g., YXXΦ motifs) on cargo proteins. Mutations in *AP2S1* are linked to familial hypocalciuric hypercalcemia type 3 (FHH3), highlighting its physiological importance in calcium homeostasis. AP2σ1 also interacts with phosphoinositides, aiding membrane localization during endocytosis.
Recombinant AP2σ1 protein is engineered for *in vitro* studies to dissect molecular mechanisms of CME, protein-protein interactions, and cargo recognition. Its production typically involves expression systems (e.g., *E. coli* or mammalian cells) followed by purification. Researchers use it to resolve structures (e.g., via cryo-EM), map binding interfaces, or screen small molecules targeting endocytic pathways.
AP2σ1 recombinant protein has become a vital tool in cell biology, offering insights into trafficking disorders, neurological diseases, and cancer, where dysregulated endocytosis contributes to pathogenesis. Its study also aids in developing therapeutics modulating endocytic pathways, such as targeted drug delivery systems.
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