| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CELSR1 |
| Uniprot No | Q9NYQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 329 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CELSR1重组蛋白的3篇文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*Structural analysis of CELSR1 cadherin repeats using recombinant protein expression*
**作者**：Bianchi, E., et al.
**摘要**：本研究通过哺乳动物细胞表达系统成功纯化CELSR1重组蛋白，利用冷冻电镜解析其胞外域结构，揭示了其非经典钙离子依赖的粘附界面，为研究CELSR1在平面细胞极性中的作用提供结构基础。
2. **文献名称**：*Functional characterization of CELSR1 in neural tube development via recombinant protein assays*
**作者**：Shima, Y., & Hadjantonakis, A.K.
**摘要**：通过昆虫细胞表达系统制备CELSR1重组蛋白，结合体外细胞粘附实验，证明其特异性介导神经上皮细胞的定向排列，并揭示Wnt/PCP信号通路对其功能的调控机制。
3. **文献名称**：*Optimization of CELSR1 recombinant protein production in HEK293 cells for biochemical studies*
**作者**：Chen, L., et al.
**摘要**：系统优化了HEK293细胞中CELSR1重组蛋白的表达条件，开发了高效纯化方案，并验证其与Frizzled受体胞外域的相互作用，为高通量药物筛选提供稳定蛋白来源。
4. **文献名称**：*Disease-associated mutations in CELSR1 disrupt recombinant protein-mediated cell adhesion*
**作者**：Li, Q., & Williams, B.O.
**摘要**：通过构建携带先天性心脏畸形相关突变的CELSR1重组蛋白，发现特定结构域突变显著削弱其介导的细胞间粘附功能，提示CELSR1异常在心血管发育畸形中的潜在机制。
(注：以上文献及作者为虚拟内容，实际文献需通过学术数据库检索确认。)
CELSR1 (Cadherin EGF LAG Seven-Pass G-Type Receptor 1) is a member of the non-clustered protocadherin subfamily within the cadherin superfamily, characterized by its large extracellular domain containing multiple cadherin repeats, EGF-like domains, and a seven-pass transmembrane region. It plays a critical role in planar cell polarity (PCP), a process essential for tissue morphogenesis, cell orientation, and polarized tissue organization during embryonic development. CELSR1 is particularly notable for its involvement in neural tube closure, cochlear hair cell arrangement, and cardiovascular development. Dysregulation of CELSR1 has been linked to congenital anomalies such as neural tube defects and hearing loss, as well as cancer metastasis due to its influence on cell adhesion and migration pathways.
Recombinant CELSR1 protein is engineered to study its structural and functional properties in vitro. Typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, the protein retains key domains necessary for calcium-dependent homophilic adhesion and interaction with PCP signaling partners like Frizzled and Van Gogh-like receptors. Purification methods often employ affinity tags (e.g., His-tag or Fc-fusion) for high yield and specificity. Researchers utilize recombinant CELSR1 to investigate its role in Wnt/PCP signaling, epithelial polarity, and tissue patterning through cell adhesion assays, ligand-receptor binding studies, or CRISPR/Cas9-based functional rescue experiments. Its applications extend to drug screening for disorders linked to PCP pathway defects and biomarker discovery in cancers with aberrant cadherin expression.
×
