| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP1s1 |
| Uniprot No | P61966 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-158aa |
| 氨基酸序列 | MMRFMLLFSR QGKLRLQKWY LATSDKERKK MVRELMQVVL ARKPKMCSFL EWRDLKVVYK RYASLYFCCA IEGQDNELIT LELIHRYVEL LDKYFGSVCE LDIIFNFEKA YFILDEFLMG GDVQDTSKKS VLKAIEQADL LQEEDESPRS VLEEMGLA |
| 预测分子量 | 18,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP1S1重组蛋白的3篇参考文献(示例为模拟内容，非真实文献)：
1. **文献名称**：*AP1S1 mutations impair cellular trafficking and cause MEDNIK syndrome through disrupted adaptor complex assembly*
**作者**：Montpetit A, et al.
**摘要**：研究揭示了AP1S1基因突变导致细胞内高尔基体-溶酶体运输缺陷，通过重组AP1S1蛋白体外实验证实突变体无法与AP1复合物其他亚基结合，阐明了MEDNIK综合征的分子机制。
2. **文献名称**：*Recombinant AP1S1 facilitates clathrin-coated vesicle formation in vitro*
**作者**：Boehm M, Bonifacino JS
**摘要**：报道了人源AP1S1重组蛋白的异源表达与纯化方法，证明其在体外促进AP1复合物与网格蛋白的结合，揭示了其在细胞内膜运输中的关键作用。
3. **文献名称**：*Structural analysis of AP1S1 reveals cargo recognition specificity in polarized sorting*
**作者**：Hirst J, et al.
**摘要**：通过晶体学解析AP1S1重组蛋白结构，发现其C端结构域特异性识别跨膜蛋白的酪氨酸分选信号，为理解上皮细胞极性建立提供了分子基础。
注：以上为基于AP1复合物相关研究的模拟内容，实际文献需通过PubMed/Google Scholar以"AP1S1 recombinant protein"等关键词检索获取。
**Background of AP1S1 Recombinant Protein**
AP1S1 (Adaptor Protein complex 1 subunit sigma 1) is a critical component of the heterotetrameric AP-1 complex, which facilitates intracellular vesicular trafficking between the trans-Golgi network (TGN) and endosomes. As part of the adaptor protein (AP) family, AP-1 plays a key role in clathrin-mediated cargo sorting, membrane deformation, and vesicle formation. The AP1S1 subunit, a 19 kDa protein, contributes to the recognition of tyrosine-based sorting signals on transmembrane cargos, ensuring their proper packaging into transport vesicles.
Mutations in the *AP1S1* gene are linked to several human disorders, including MEDNIK syndrome (Mental retardation, Enteropathy, Deafness, peripheral Neuropathy, Ichthyosis, and Keratoderma), a rare autosomal recessive condition characterized by multisystemic defects. These mutations disrupt AP-1 function, impairing protein trafficking and leading to cellular dysregulation in tissues such as the skin, intestine, and nervous system.
Recombinant AP1S1 protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to study its structural and functional roles. It serves as a tool for investigating AP-1 complex assembly, cargo-binding specificity, and interactions with clathrin or accessory proteins. Additionally, recombinant AP1S1 aids in elucidating pathogenic mechanisms of trafficking-related diseases and screening potential therapeutic agents. Its applications extend to structural biology (e.g., crystallography) and reconstituting vesicle formation assays in cell-free systems.
Research on AP1S1 recombinant protein continues to advance understanding of intracellular trafficking pathways and their implications in genetic and acquired diseases.
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