| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP11B |
| Uniprot No | Q9Y2G3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1177aa |
| 氨基酸序列 | MWRWIRQQLGFDPPHQSDTRTIYVANRFPQNGLYTPQKFIDNRIISSKYTVWNFVPKNLFEQFRRVANFYFLIIFLVQLMIDTPTSPVTSGLPLFFVITVTAIKQGYEDWLRHNSDNEVNGAPVYVVRSGGLVKTRSKNIRVGDIVRIAKDEIFPADLVLLSSDRLDGSCHVTTASLDGETNLKTHVAVPETALLQTVANLDTLVAVIECQQPEADLYRFMGRMIITQQMEEIVRPLGPESLLLRGARLKNTKEIFGVAVYTGMETKMALNYKSKSQKRSAVEKSMNTFLIIYLVILISEAVISTILKYTWQAEEKWDEPWYNQKTEHQRNSSKILRFISDFLAFLVLYNFIIPISLYVTVEMQKFLGSFFIGWDLDLYHEESDQKAQVNTSDLNEELGQVEYVFTDKTGTLTENEMQFRECSINGMKYQEINGRLVPEGPTPDSSEGNLSYLSSLSHLNNLSHLTTSSSFRTSPENETELIKEHDLFFKAVSLCHTVQISNVQTDCTGDGPWQSNLAPSQLEYYASSPDEKALVEAAARIGIVFIGNSEETMEVKTLGKLERYKLLHILEFDSDRRRMSVIVQAPSGEKLLFAKGAESSILPKCIGGEIEKTRIHVDEFALKGLRTLCIAYRKFTSKEYEEIDKRIFEARTALQQREEKLAAVFQFIEKDLILLGATAVEDRLQDKVRETIEALRMAGIKVWVLTGDKHETAVSVSLSCGHFHRTMNILELINQKSDSECAEQLRQLARRITEDHVIQHGLVVDGTSLSLALREHEKLFMEVCRNCSAVLCCRMAPLQKAKVIRLIKISPEKPITLAVGDGANDVSMIQEAHVGIGIMGKEGRQAARNSDYAIARFKFLSKLLFVHGHFYYIRIATLVQYFFYKNVCFITPQFLYQFYCLFSQQTLYDSVYLTLYNICFTSLPILIYSLLEQHVDPHVLQNKPTLYRDISKNRLLSIKTFLYWTILGFSHAFIFFFGSYLLIGKDTSLLGNGQMFGNWTFGTLVFTVMVITVTVKMALETHFWTWINHLVTWGSIIFYFVFSLFYGGILWPFLGSQNMYFVFIQLLSSGSAWFAIILMVVTCLFLDIIKKVFDRHLHPTSTEKAQLTETNAGIKCLDSMCCFPEGEAACASVGRMLERVIGRCSPTHISRSWSASDPFYTNDRSILTLSTMDSSTC |
| 预测分子量 | 134,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP11B重组蛋白的假设性参考文献示例(请注意，这些文献信息为虚构，仅用于演示格式)：
1. **文献名称**: "Recombinant Expression and Functional Characterization of Human ATP11B Phospholipid Flippase"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在昆虫细胞中表达了重组ATP11B蛋白，并验证其具有ATP依赖的磷脂翻转酶活性，揭示了其在细胞膜不对称性维持中的关键作用。
2. **文献名称**: "Structural Insights into ATP11B by Cryo-EM: Implications for Substrate Specificity"
**作者**: Tanaka K, et al.
**摘要**: 通过冷冻电镜解析了重组ATP11B蛋白的高分辨率结构，发现其跨膜结构域独特的电荷分布可能决定其对特定磷脂底物的选择性转运。
3. **文献名称**: "ATP11B Knockdown and Recombinant Rescue in Cancer Cell Migration Studies"
**作者**: Gupta R, et al.
**摘要**: 利用重组ATP11B蛋白回复实验，证明其在乳腺癌细胞中通过调节膜磷脂分布影响细胞迁移能力，为靶向治疗提供依据。
4. **文献名称**: "Optimizing ATP11B Recombinant Production in Mammalian Expression Systems"
**作者**: Müller S, et al.
**摘要**: 系统比较了HEK293和CHO细胞中ATP11B重组表达的效率，开发出新型去垢剂纯化方案，显著提高蛋白产量及稳定性。
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实际文献需通过PubMed/Google Scholar以关键词“ATP11B recombinant”检索，建议结合具体研究方向筛选。
**Background of ATP11B Recombinant Protein**
ATP11B is a member of the P4-type ATPase family, a group of transmembrane enzymes critical for maintaining phospholipid asymmetry in biological membranes. These proteins act as lipid flippases, utilizing energy from ATP hydrolysis to translocate specific phospholipids (e.g., phosphatidylserine) from the outer to the inner leaflet of cell membranes. This activity is essential for cellular processes such as vesicle trafficking, membrane repair, and apoptosis regulation.
The ATP11B gene, located on human chromosome 3. encodes a multi-pass membrane protein predominantly expressed in tissues like the brain, liver, and testes. Dysregulation of ATP11B has been linked to neurological disorders, cancer progression, and metabolic diseases, highlighting its physiological importance. Recombinant ATP11B protein is engineered using heterologous expression systems (e.g., mammalian or insect cell lines) to ensure proper post-translational modifications and functional fidelity.
Purified recombinant ATP11B is widely used in biochemical studies to dissect its flippase activity, substrate specificity, and interaction partners. Its structure-function relationship is explored via mutagenesis or cryo-EM to identify critical domains for lipid binding and ATP hydrolysis. Additionally, this protein serves as a tool in drug discovery, enabling screens for modulators targeting membrane-related pathologies. Research on ATP11B also contributes to understanding broader mechanisms of cellular membrane homeostasis and disease-associated lipid dysregulation.
In summary, ATP11B recombinant protein is a vital reagent for unraveling the molecular basis of lipid transport and its implications in health and disease.
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