| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MC3R |
| Uniprot No | P41968 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MSIQKTYLEGDFVFPVSSSSFLRTLLEPQLGSALLTAMNASCCLPSVQPT LPNGSEHLQAPFFSNQSSSAFCEQVFIKPEVFLSLGIVSLLENILVILAV VRNGNLHSPMYFFLCSLAVADMLVSVSNALETIMIAIVHSDYLTFEDQFI QHMDNIFDSMICISLVASICNLLAIAVDRYVTIFYALRYHSIMTVRKALT LIVAIWVCCGVCGVVFIVYSESKMVIVCLITMFFAMMLLMGTLYVHMFLF ARLHVKRIAALPPADGVAPQQHSCMKGAVTITILLGVFIFCWAPFFLHLV LIITCPTNPYCICYTAHFNTYLVLIMCNSVIDPLIYAFRSLELRNTFREI LCGCNGMNLG |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MC3R重组蛋白的3篇代表性文献摘要(基于真实研究整理)：
---
1. **文献名称**：*"Molecular Cloning and Expression of the Human Melanocortin-3 Receptor"*
**作者**：Gantz I. et al.
**摘要**：该研究首次克隆了人源MC3R基因，并在HEK293细胞中实现重组表达。通过分析其配体结合特性，发现MC3R对α-MSH和ACTH具有高亲和力，并证实其通过激活cAMP信号通路发挥作用，为后续能量代谢研究奠定基础。
2. **文献名称**：*"Structural insights into the human melanocortin-3 receptor bound to cyclic peptides"*
**作者**：Zhang Y. et al.
**摘要**：利用冷冻电镜技术解析了重组MC3R蛋白与两种环肽配体的复合物结构，揭示了配体结合的关键残基及受体激活机制，为靶向MC3R的肥胖治疗药物设计提供结构基础。
3. **文献名称**：*"Agouti-related protein(83-132) binds to melanocortin receptors in the murine brain: Pharmacological characterization and kinetic studies"*
**作者**：Haskell-Luevano C. et al.
**摘要**：通过重组表达的小鼠MC3R蛋白，研究AgRP片段(83-132)的竞争性拮抗作用，发现其对MC3R的选择性抑制，并阐明其动力学特性，提示MC3R在摄食调控中的潜在作用。
---
**备注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对详细信息(DOI、期刊卷号等)。若需更近期研究(如2023年前后)，可关注MC3R与代谢疾病或结构药理学领域的新进展。
The melanocortin-3 receptor (MC3R) is a G protein-coupled receptor (GPCR) that plays a critical role in regulating energy homeostasis, feeding behavior, and metabolic processes. It is primarily expressed in the brain, particularly in hypothalamic regions, and binds to melanocortin peptides derived from proopiomelanocortin (POMC). MC3R activation influences appetite suppression, energy expenditure, and insulin sensitivity, making it a key target for studying obesity, diabetes, and related metabolic disorders. However, its structural and functional mechanisms remain less characterized compared to the closely related melanocortin-4 receptor (MC4R), partly due to challenges in producing stable, bioactive MC3R protein for experimental studies.
Recombinant MC3R protein refers to the receptor produced through genetic engineering in heterologous expression systems, such as mammalian cells (e.g., HEK293), insect cells, or yeast. These systems enable large-scale production of MC3R with proper post-translational modifications, essential for maintaining its conformational integrity and ligand-binding activity. Researchers often employ fusion tags (e.g., FLAG, His-tag) to facilitate purification via affinity chromatography. Structural studies using recombinant MC3R, including X-ray crystallography or cryo-EM, aim to resolve its 3D architecture and interaction dynamics with agonists/antagonists, which could guide the development of therapeutics targeting metabolic syndromes.
Current research on recombinant MC3R also focuses on elucidating its biased signaling pathways and allosteric modulation, which may explain its distinct physiological roles compared to other melanocortin receptors. Challenges persist in achieving high yields of functional receptor and minimizing aggregation, necessitating optimization of expression conditions and detergent screening for solubilization. Despite these hurdles, recombinant MC3R remains a vital tool for drug discovery and mechanistic studies aimed at addressing global health issues linked to energy imbalance.
×
