| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPRR2A |
| Uniprot No | P35326 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-72aa |
| 氨基酸序列 | MSYQQQQCKQ PCQPPPVCPT PKCPEPCPPP KCPEPCPPPK CPQPCPPQQC QQKYPPVTPS PPCQSKYPPK SK |
| 预测分子量 | 7,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPRR2A重组蛋白的3篇参考文献示例(内容为模拟概括，建议通过学术数据库核实真实文献)：
1. **文献名称**：*SPRR2A重组蛋白在皮肤屏障功能中的作用机制研究*
**作者**：Zhang L, et al.
**摘要**：本研究通过大肠杆菌表达系统成功制备了重组SPRR2A蛋白，并发现其在体外模型中能够促进角质形成细胞分化，增强细胞膜稳定性。结果表明，SPRR2A可能通过调控表皮分化相关蛋白(如loricrin)的表达参与皮肤屏障形成。
2. **文献名称**：*重组SPRR2A蛋白对上皮细胞氧化应激反应的调控作用*
**作者**：Wang X, et al.
**摘要**：通过纯化SPRR2A重组蛋白，研究发现其在人永生化角质形成细胞(HaCaT)中能够显著降低过氧化氢诱导的ROS水平，并激活Nrf2抗氧化通路。提示SPRR2A可能通过抗氧化机制参与皮肤炎症修复过程。
3. **文献名称**：*SPRR2A重组蛋白与食管鳞状细胞癌迁移能力的相关性分析*
**作者**：Chen Y, et al.
**摘要**：利用真核表达系统获得高纯度SPRR2A重组蛋白，实验显示其可通过抑制EGFR信号通路显著降低食管癌细胞(KYSE-150)的侵袭迁移能力，为SPRR家族蛋白在肿瘤转移中的功能研究提供了新证据。
(注：以上文献信息为学术问题解答示例，具体研究请参考PubMed、Web of Science等平台的最新文献)
SPRR2A (Small Proline-Rich Protein 2A) is a member of the SPRR family, a group of cysteine-rich, cross-linked envelope proteins predominantly expressed in stratified squamous epithelia, particularly the skin. These proteins play critical roles in epidermal differentiation and barrier formation. SPRR2A is encoded within the epidermal differentiation complex (EDC) on chromosome 1 and is upregulated during terminal keratinocyte differentiation. It contributes to the assembly of the cornified cell envelope (CE), a resilient structure that protects against mechanical stress, pathogens, and dehydration.
Structurally, SPRR2A contains a central proline-rich domain flanked by conserved peptide motifs, enabling interactions with other CE components like loricrin, involucrin, and transglutaminases. Its expression is tightly regulated by environmental cues (e.g., UV radiation) and signaling pathways (e.g., p63. Notch). Dysregulation of SPRR2A has been implicated in skin disorders such as psoriasis, atopic dermatitis, and squamous cell carcinoma, where abnormal keratinocyte differentiation or proliferation occurs.
Recombinant SPRR2A protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), is widely used to study its biochemical properties, protein-protein interactions, and role in epidermal homeostasis. It also serves as an antigen in immunological studies, given its potential autoantigenic role in certain dermatological conditions. Recent research explores its utility in tissue engineering and regenerative medicine, leveraging its structural role in barrier formation. Despite its small size (~10-15 kDa), SPRR2A’s functional versatility makes it a key focus in dermatological and epithelial biology research.
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