| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | AP1m2 |
| Uniprot No | Q9WVP1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MSASAVFILD VKGKPLISRN YKGDVPMTEI DHFMPLLMQR EEEGVLAPLL SHGRVHFLWI KHSNLYLVAT TLKNANASLV YSFLYKTVEV FCEYFKELEE ESIRDNFVIV YDLLDELMDF GFPQTTDSKI LQEYITQQGN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ CVRLSRFDND RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPEKNV VIWSIKSFPG GKEYLMRAHF GLPSVETEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK SGYQALPWVR YITQSGDYQL RTS |
| 预测分子量 | 48,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP1M2重组蛋白的3篇参考文献示例：
---
1. **标题**: "AP1M2 regulates Wnt signaling and controls melanoma metastasis"
**作者**: Smith, J., et al.
**摘要**: 研究通过表达AP1M2重组蛋白，揭示其通过调控Wnt/β-catenin信号通路促进黑色素瘤细胞转移的机制，为靶向AP1M2的癌症治疗提供依据。
---
2. **标题**: "Structural and functional analysis of AP1M2 in polarized epithelial cells"
**作者**: Tanaka, K., et al.
**摘要**: 利用重组AP1M2蛋白进行体外结合实验，证明其与细胞极性蛋白相互作用，对上皮细胞顶端-基底极性形成至关重要，并解析了关键结构域。
---
3. **标题**: "AP1M2 mutations impair protein trafficking in lysosomal storage disorders"
**作者**: Garcia, R., et al.
**摘要**: 研究构建AP1M2突变体重组蛋白，发现其异常导致溶酶体运输缺陷，与特定溶酶体贮积症相关，为疾病机制提供了分子层面的解释。
---
(注：以上文献为示例，实际引用时需核实真实存在的论文。)
AP1M2 (Adaptor Protein Complex 1 Subunit Mu-2) is a subunit of the heterotetrameric AP-1 complex, which plays a critical role in intracellular vesicle trafficking and cargo sorting. The AP-1 complex, composed of β1. γ1. μ1. and σ1 subunits, facilitates the formation of clathrin-coated vesicles and mediates protein transport between the trans-Golgi network (TGN) and endosomes. AP1M2. a paralog of the ubiquitously expressed AP1M1. exhibits tissue-specific expression, predominantly in polarized epithelial cells and specialized secretory cells. Its unique structural features, including a conserved phosphoinositide-binding domain, enable selective interactions with membrane lipids and cargo receptors, ensuring precise spatial sorting of proteins like receptors, enzymes, and adhesion molecules.
The recombinant AP1M2 protein is engineered using recombinant DNA technology, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications. Purification typically involves affinity chromatography tags (e.g., His-tag) followed by functional validation via lipid-binding assays or co-immunoprecipitation with known interactors.
Research on AP1M2 has highlighted its role in maintaining epithelial polarity, neurosecretory processes, and immune regulation. Dysregulation of AP1M2 is linked to cancers (e.g., breast, cervical) due to its involvement in metastatic pathways and extracellular matrix remodeling. Recombinant AP1M2 serves as a vital tool for dissecting trafficking mechanisms, modeling diseases, and screening therapeutic compounds targeting membrane trafficking disorders. Its study bridges fundamental cell biology with translational applications in oncology and genetic diseases.
×
