| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FUT5 |
| Uniprot No | Q11128 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-374aa |
| 氨基酸序列 | MDPLGPAKPQWLWRRCLAGLLFQLLVAVCFFSYLRVSRDDATGSPRPGLMAVEPVTGAPNGSRCQDSMATPAHPTLLILLWTWPFNTPVALPRCSEMVPGAADCNITADSSVYPQADAVIVHHWDIMYNPSANLPPPTRPQGQRWIWFSMESPSNCRHLEALDGYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFHWRETLRPRSFSWALAFCKACWKLQQESRYQTVRSIAAWFT |
| 预测分子量 | 42,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FUT5重组蛋白的示例参考文献(内容为模拟示例，非真实文献)：
---
1. **文献名称**：*Expression and Characterization of Recombinant Human FUT5 in HEK293 Cells*
**作者**：Kudo T, et al.
**摘要**：本研究在HEK293细胞中成功表达了重组人源FUT5蛋白，通过亲和层析纯化后分析了其酶活性。结果表明，重组FUT5对Lewis X抗原合成具有高特异性，为研究其在肿瘤细胞黏附中的作用提供了工具。
2. **文献名称**：*Functional Analysis of FUT5 in Bacterial Adhesion Using a Prokaryotic Expression System*
**作者**：Costa AF, et al.
**摘要**：利用大肠杆菌系统表达并纯化功能性FUT5重组蛋白，发现其催化合成的糖结构可增强致病菌与宿主细胞的结合能力，揭示了FUT5在感染机制中的潜在作用。
3. **文献名称**：*Structural Insights into FUT5 Substrate Specificity via Crystallography*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析了重组FUT5的三维结构，阐明了其与GDP-岩藻糖及受体底物的结合模式，为开发针对FUT5的抑制剂提供了结构基础。
4. **文献名称**：*Optimization of FUT5 Recombinant Production in Insect Cells for Glycoengineering*
**作者**：Sørensen AL, et al.
**摘要**：在昆虫细胞中优化了FUT5重组蛋白的高效表达，证明其可用于体外糖链修饰工程，显著提高抗体药物的靶向性。
---
注：以上文献为示例，实际研究需通过学术数据库(如PubMed、Web of Science)检索真实文献。
FUT5 (Fucosyltransferase 5) is a member of the α1.3-fucosyltransferase family that catalyzes the transfer of fucose from GDP-fucose to acceptor glycoconjugates via α1.3-linkages. It plays a critical role in synthesizing Lewis blood group antigens (e.g., Lewis X and sialyl Lewis X) on cell surfaces, which are involved in cell-cell recognition, immune responses, and pathogen adhesion. FUT5 is expressed in epithelial tissues, leukocytes, and certain tumors, with dysregulation linked to cancer metastasis, chronic inflammation, and microbial infections.
Recombinant FUT5 protein is typically produced using heterologous expression systems like mammalian (e.g., CHO cells) or bacterial hosts (e.g., E. coli). Mammalian systems are preferred for generating enzymatically active FUT5 with proper post-translational modifications, while bacterial systems offer cost-effective production of truncated forms for structural studies. The purified protein retains catalytic activity to modify glycan structures in vitro, making it valuable for glycobiology research. Applications include studying glycosylation patterns in disease models, developing glycan-based diagnostics, and exploring therapeutic strategies targeting fucose-dependent interactions (e.g., blocking pathogen adhesion or inhibiting tumor cell migration).
Current challenges in recombinant FUT5 production involve maintaining enzyme stability, optimizing substrate specificity, and scaling up functional yields. Advances in glycoengineering and structural analysis continue to refine its applications in biomedicine and industrial glycoconjugate synthesis.
×
