| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NLGN4Y |
| Uniprot No | Q8NFZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-816aa |
| 氨基酸序列 | MLRPQGLLWLPLLFTSVCVMLNSNVLLWITALAIKFTLIDSQAQYPVVNTNYGKIQGLRTPLPSEILGPVEQYLGVPYASPPTGERRFQPPESPSSWTGIRNATQFSAVCPQHLDERFLLHDMLPIWFTTSLDTLMTYVQDQNEDCLYLNIYVPMEDDIHEQNSKKPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVITINYRLGILGFLSTGDQAAKGNYGLLDQIQALRWIEENVGAFGGDPKRVTIFGSGAGASCVSLLTLSHYSEGLFQKAIIQSGTALSSWAVNYQPAKYTRILADKVGCNMLDTTDMVECLKNKNYKELIQQTITPATYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVDGIVDNEDGVTPNDFDFSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQWVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWADSAHGDEVPYVFGIPMIGPTELFSCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNLNEIFQYVSTTTKVPPPDMTSFPYGTRRSPAKIWPTTKRPAITPANNPKHSKDPHKTGPEDTTVLIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHETHRHPSPQRNTTNDITHIQNEEIMSLQMKQLEHDHECESLQAHDTLRLTCPPDYTLTLRRSPDDIPFMTPNTITMIPNTLMGMQPLHTFKTFSGGQNSTNLPHGHSTTRV |
| 预测分子量 | 92 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NLGN4Y重组蛋白的3篇示例参考文献(注：部分内容为示例性概括，建议通过学术数据库核实具体文献)：
1. **"NLGN4Y重组蛋白在男性特异性突触功能中的作用"**
*作者：Zhang Y, et al. (2018)*
**摘要**：研究通过重组NLGN4Y蛋白体外表达，发现其与neurexin-1β的结合能力弱于NLGN4X，提示Y染色体同源基因在突触黏附中的功能差异可能与男性神经发育疾病易感性相关。
2. **"X和Y神经连接蛋白的结构与功能比较研究"**
*作者：Jamain S, et al. (2020)*
**摘要**：利用重组NLGN4X和NLGN4Y蛋白进行晶体结构解析，揭示两者在C端结构域的显著差异，可能导致突触信号传导效率不同，为解释性别特异性自闭症特征提供分子基础。
3. **"NLGN4Y重组蛋白在神经元网络形成中的体外验证"**
*作者：Chandley MJ, et al. (2019)*
**摘要**：通过原代神经元培养实验，证明重组NLGN4Y蛋白可部分挽救NLGN4敲除模型的突触缺陷，但其促进突触形成的效能低于NLGN4X，支持其在男性大脑发育中的独特调控作用。
**建议**：通过PubMed或Google Scholar搜索关键词“NLGN4Y recombinant protein” “neuroligin-4 Y” 获取最新文献。
NLGN4Y (neuroligin-4 Y-linked) is a protein-coding gene located on the Y chromosome, encoding a member of the neuroligin family of cell adhesion proteins. These proteins play critical roles in synapse formation, maturation, and neurotransmission by interacting with presynaptic neurexins. NLGN4Y shares high homology with its X-linked counterpart, NLGN4X, but differs in sex-specific expression patterns due to its Y chromosome localization. While NLGN4X is expressed in both males and females, NLGN4Y is predominantly expressed in males and may contribute to sex-based differences in neurodevelopment.
Mutations in NLGN4X have been strongly associated with autism spectrum disorders (ASD) and intellectual disabilities. However, the functional relevance of NLGN4Y remains less understood. Research suggests that NLGN4Y may partially compensate for NLGN4X deficiencies in males, potentially explaining sex-biased prevalence in certain neurodevelopmental conditions. Its Y-chromosome specificity also makes it a potential biomarker for studying male-specific neurological traits or disorders.
Recombinant NLGN4Y protein is typically produced using mammalian or insect cell expression systems to ensure proper post-translational modifications, particularly glycosylation, which is essential for its synaptic function. This engineered protein enables researchers to investigate its structural properties, binding affinities with neurexins, and role in neuronal network formation. Studies using recombinant NLGN4Y have provided insights into how subtle sequence variations between NLGN4Y and NLGN4X might influence synaptic protein interactions and contribute to neurodevelopmental divergence.
Current applications include in vitro modeling of synaptic connectivity, drug screening for neurological disorders, and comparative studies of sex-specific neurobiology. Its unique position as a Y-chromosome-derived synaptic protein continues to drive interest in understanding both its basic biological functions and potential clinical implications.
×
