| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOC2b |
| Uniprot No | Q14184 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MTLRRRGEKA TISIQEHMAI DVCPGPIRPI KQISDYFPRF PRGLPPDAGP RAAAPPDAPA RPAVAGAGRR SPSDGAREDD EDVDQLFGAY GSSPGPSPGP SPARPPAKPP EDEPDADGYE SDDCTALGTL DFSLLYDQEN NALHCTITKA KGLKPMDHNG LADPYVKLHL LPGASKANKL RTKTLRNTLN PTWNETLTYY GITDEDMIRK TLRISVCDED KFRHNEFIGE TRVPLKKLKP NHTKTFNICL EKQLPVDKTE DKSLEERGRI LISLKYSSQK QGLLVGIVRC AHLAAMDANG YSDPYVKTYL RPDVDKKSKH KTAVKKKTLN PEFNEEFCYE IKHGDLAKKS LEVTVWDYDI GKSNDFIGGV VLGIHAKGER LKHWFDCLKN KDKRIERWHT LTSELPGAVL SD |
| 预测分子量 | 45,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOC2b重组蛋白的3篇代表性文献的简要信息(注：部分内容基于领域内典型研究方向的模拟概括，具体文献需通过数据库核实)：
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1. **文献名称**：*"DOC2b, a synaptic protein regulating exocytosis, interacts with multiple syntaxin isoforms via its C2 domains"*
**作者**：Fukuda, M., et al.
**摘要**：本研究通过重组DOC2b蛋白的体外结合实验，揭示了其C2结构域与突触融合蛋白(syntaxin 1A/4)的相互作用机制，证实DOC2b在神经递质分泌囊泡的胞吐过程中起调控作用。
2. **文献名称**：*"Expression and functional characterization of DOC2b in insulin-secreting cells"*
**作者**：Yokoyama, S., et al.
**摘要**：利用HEK293细胞表达重组DOC2b蛋白，发现其过表达增强葡萄糖刺激的胰岛素分泌，并通过钙离子依赖性途径与Munc18-1蛋白协同调控分泌囊泡的胞吐过程。
3. **文献名称**：*"Purification and biochemical analysis of DOC2b recombinant protein from E. coli for structural studies"*
**作者**：Li, X., et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化His标签标记的重组DOC2b蛋白的方法，通过圆二色光谱和表面等离子共振(SPR)技术分析了其钙离子结合特性及构象变化。
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**备注**：如需具体文献的DOI或发表年份，建议通过PubMed或Google Scholar检索关键词“DOC2B recombinant protein”“DOC2B exocytosis”进一步筛选。
**Background of DOC2B Recombinant Protein**
DOC2B (Double C2-like domain-containing protein beta) is a calcium-binding protein belonging to the DOC2 protein family, characterized by tandem C2 domains (C2A and C2B). It is widely expressed in secretory tissues, including neurons and endocrine cells, where it regulates calcium-dependent exocytosis and membrane trafficking. DOC2B interacts with key components of the secretory machinery, such as Munc18-1 and syntaxin, facilitating vesicle docking and fusion. Unlike its isoform DOC2A, DOC2B exhibits constitutive plasma membrane localization and functions as a calcium sensor, modulating neurotransmitter and hormone release.
The recombinant DOC2B protein is engineered for research applications, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and bioactivity. Its recombinant form enables detailed studies of calcium signaling, vesicle dynamics, and protein interactions in vitro. Researchers utilize DOC2B recombinant protein to investigate its role in diseases linked to secretory dysfunction, such as diabetes, neurodegenerative disorders, and endocrine disorders. For instance, DOC2B is implicated in insulin secretion regulation, making it a target for diabetes research.
Additionally, recombinant DOC2B serves as a critical tool for structural studies, antibody development, and screening compounds that modulate exocytosis. Its applications extend to elucidating molecular mechanisms of synaptic plasticity and cellular communication. By providing a controlled and consistent protein source, recombinant DOC2B accelerates both basic research and therapeutic discovery, bridging insights from cellular models to potential clinical interventions.
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