| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOC2a |
| Uniprot No | Q14183 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MRGRRGDRMT INIQEHMAIN VCPGPIRPIR QISDYFPRGP GPEGGGGGGG EAPAHLVPLA LAPPAALLGA TTPEDGAEVD SYDSDDATAL GTLEFDLLYD RASCTLHCSI LRAKGLKPMD FNGLADPYVK LHLLPGACKA NKLKTKTQRN TLNPVWNEDL TYSGITDDDI THKVLRIAVC DEDKLSHNEF IGEIRVPLRR LKPSQKKHFN ICLERQVPLA SPSSMSAALR GISCYLKELE QAEQGQGLLE ERGRILLSLS YSSRRRGLLV GILRCAHLAA MDVNGYSDPY VKTYLRPDVD KKSKHKTCVK KKTLNPEFNE EFFYEIELST LATKTLEVTV WDYDIGKSND FIGGVSLGPG ARGEARKHWS DCLQQPDAAL ERWHTLTSEL PPAAGALSSA |
| 预测分子量 | 43,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOC2a重组蛋白的3篇代表性文献的简化示例(部分信息为模拟生成，仅供参考)：
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1. **文献名称**: *DOC2a regulates synaptic vesicle priming and interacts with RIM1α in neurotransmitter release*
**作者**: Verhage, M. et al.
**摘要**: 研究通过重组DOC2a蛋白，揭示其与突触前蛋白RIM1α的相互作用，证明DOC2a在囊泡启动和钙依赖性神经递质释放中的关键作用。
2. **文献名称**: *Expression and functional characterization of recombinant DOC2a in neuroendocrine cells*
**作者**: Yao, J. et al.
**摘要**: 报道DOC2a重组蛋白在大肠杆菌中的高效表达与纯化，并利用体外实验验证其C2结构域对钙离子的结合能力及囊泡融合的调控机制。
3. **文献名称**: *DOC2 isoforms play distinct roles in asynchronous neurotransmitter release*
**作者**: Groffen, A.J. et al.
**摘要**: 通过比较DOC2a与DOC2b重组蛋白的功能差异，发现DOC2a特异性参与延迟性突触传递，依赖其N端结构域与磷脂膜的相互作用。
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**注**：以上文献标题及内容为示例性质，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“DOC2a recombinant”、“DOC2a exocytosis”或结合具体研究领域(如神经科学、蛋白表达)筛选最新论文。
DOC2a (Double C2-like domain protein alpha) is a calcium-sensitive protein belonging to the DOC2 protein family, which is primarily expressed in neurons and neuroendocrine cells. It contains two C2 domains (C2A and C2B) that mediate calcium-dependent interactions with membranes and partner proteins. DOC2a plays a critical role in regulating synaptic vesicle exocytosis and neurotransmitter release. Unlike its isoform DOC2b, which is ubiquitously expressed, DOC2a is specifically enriched in the brain and functions as a calcium sensor that facilitates synaptic plasticity. It interacts with key exocytosis regulators, such as Munc13 and RIM1. to modulate the priming and fusion of synaptic vesicles with the presynaptic membrane. Calcium influx triggers DOC2a’s translocation from the cytoplasm to the plasma membrane, promoting vesicle docking and fusion via its association with the SNARE complex.
Recombinant DOC2a protein is engineered for in vitro studies to dissect molecular mechanisms underlying neurotransmitter release, synaptic transmission, and calcium signaling. Researchers use it to investigate DOC2a’s structural domains, calcium-binding kinetics, and interactions with lipid bilayers or effector proteins. Its recombinant form allows precise control over purity and concentration, eliminating confounding factors in cellular assays. Studies involving DOC2a have implications for understanding neurodevelopmental disorders, neurodegenerative diseases (e.g., Alzheimer’s), and psychiatric conditions linked to synaptic dysfunction. For instance, DOC2a mutations or dysregulation are associated with epilepsy and cognitive deficits, highlighting its potential as a therapeutic target. By leveraging recombinant DOC2a, scientists aim to develop targeted interventions for neurological diseases and advance foundational knowledge of synaptic biology.
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