| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP2b1 |
| Uniprot No | Q10567 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-584aa |
| 氨基酸序列 | MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTALNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTLLKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRKYPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVKLFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLLDELICYIGTLASVYHKPPSAFVEG |
| 预测分子量 | 73.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP2B1重组蛋白的3篇代表性文献信息及摘要概括：
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1. **文献名称**: *"Adaptor protein complex AP-2 regulates lysosomal degradation of endocytosed EGFR"*
**作者**: Chen, H., et al.
**摘要**: 该研究揭示了AP2B1作为AP-2复合体的β亚基，在调控表皮生长因子受体(EGFR)内吞及溶酶体降解中的关键作用。通过重组蛋白实验，发现AP2B1缺失导致EGFR内吞障碍，并影响下游信号通路。
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2. **文献名称**: *"Structural basis of AP2B1 recognition by the autophagy receptor TBC1D5"*
**作者**: Vargas, J.N.S., et al.
**摘要**: 利用重组AP2B1蛋白进行结构解析，阐明了其与自噬相关蛋白TBC1D5的相互作用机制，揭示了AP2B1在自噬-内吞交叉调控中的分子基础。
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3. **文献名称**: *"AP2B1 variants associated with neurodevelopmental disorders disrupt endosomal trafficking"*
**作者**: Lelieveld, S.H., et al.
**摘要**: 通过构建AP2B1重组蛋白突变体，研究证明神经发育障碍相关突变会破坏内吞体运输功能，为AP2B1基因异常导致疾病的机制提供了实验依据。
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**备注**：以上文献信息为示例性质，具体内容需根据实际发表的论文调整。建议通过PubMed或Google Scholar以“AP2B1 recombinant protein”为关键词检索最新研究。
**Background of AP2B1 Recombinant Protein**
The AP2B1 (Adaptor Protein 2 Beta 1) recombinant protein is derived from the AP2B1 gene, which encodes the β-subunit of the Adaptor Protein 2 (AP-2) complex. This evolutionarily conserved heterotetrameric complex, composed of α, β2. μ2. and σ2 subunits, plays a central role in clathrin-mediated endocytosis (CME), a critical process for internalizing membrane-bound receptors, nutrients, and signaling molecules. The AP-2 complex acts as a scaffold, bridging clathrin coats to cargo proteins and lipids, while also recruiting accessory proteins to orchestrate vesicle formation.
AP2B1. the β2 subunit, contributes to the structural stability of the AP-2 complex and facilitates interactions with tyrosine-based sorting motifs in cargo proteins. It is essential for cellular processes such as synaptic vesicle recycling, receptor trafficking, and maintaining plasma membrane homeostasis. Dysregulation of AP2B1 has been implicated in neurological disorders, cancer, and metabolic syndromes, highlighting its biological and therapeutic relevance.
Recombinant AP2B1 protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., His-tag). It serves as a vital tool for *in vitro* studies, including protein-protein interaction assays, structural analysis (e.g., crystallography or cryo-EM), and functional investigations into endocytic mechanisms. Researchers also utilize AP2B1 recombinant protein to explore disease-associated mutations or screen potential modulators of CME, offering insights into therapeutic strategies targeting membrane trafficking pathways. Its application extends to antibody development, biomarker discovery, and elucidating the molecular basis of pathologies linked to endocytic dysfunction.
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