| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP3s2 |
| Uniprot No | P59780 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-193aa |
| 氨基酸序列 | MIQAILVFNN HGKPRLVRFY QRFPEEIQQQ IVRETFHLVL KRDDNICNFL EGGSLIGGSD YKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC FENVCELDLI FHMDKVHYIL QEVVMGGMVL ETNMNEIVAQ IEAQNRLEKS EGGLSAAPAR AVSAVKNINL PEIPRNINIG DLNIKVPNLS QFV |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于AP3S2重组蛋白的参考文献(非真实文献，仅供示例参考)：
1. **文献名称**：*Expression and Purification of Recombinant AP3S2 in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：报道了AP3S2基因在大肠杆菌中的高效表达及镍柱纯化策略，获得高纯度重组蛋白用于抗体开发。
2. **文献名称**：*AP3S2 Regulates Lysosomal Protein Trafficking via AP-3 Complex Assembly*
**作者**：Chen L, et al.
**摘要**：通过体外重组AP3S2蛋白实验，揭示其在AP-3复合体组装中的关键作用及对溶酶体运输通路的影响。
3. **文献名称**：*Structural Analysis of AP3S2 Reveals a Novel σ Subunit Motif*
**作者**：Wang Y, et al.
**摘要**：利用X射线晶体学解析AP3S2重组蛋白的三维结构，发现其独特的结构域可能参与货物识别。
4. **文献名称**：*AP3S2 Dysfunction in Neuronal Models of Hermansky-Pudlak Syndrome*
**作者**：Garcia R, et al.
**摘要**：构建AP3S2重组突变体，证明其缺陷导致神经元内囊泡运输异常，与遗传性溶酶体疾病相关。
(注：以上为模拟内容，实际文献需通过PubMed/Google Scholar检索确认。)
**Background of AP3S2 Recombinant Protein**
The AP3S2 (Adaptor-Related Protein Complex 3 Subunit Sigma 2) is a critical component of the heterotetrameric AP-3 complex, which plays a pivotal role in intracellular vesicle formation and cargo sorting. The AP-3 complex, comprising β3. δ3. μ3. and σ3 (AP3S2) subunits, facilitates protein trafficking between the trans-Golgi network (TGN) and endosomal-lysosomal compartments. Specifically, AP3S2 contributes to the recognition of tyrosine-based sorting signals on cargo proteins, enabling their selective packaging into vesicles destined for lysosomes, lysosome-related organelles (LROs), and specialized secretory vesicles.
AP3S2 dysfunction is linked to genetic disorders such as Hermansky-Pudlak syndrome (HPS) type 10. characterized by impaired biogenesis of LROs, leading to oculocutaneous albinism, bleeding disorders, and immune deficiencies. Studies in model organisms and human cells highlight AP3S2's role in melanosome maturation, platelet dense granule formation, and synaptic vesicle regulation in neurons.
Recombinant AP3S2 protein, typically produced via bacterial or mammalian expression systems, is a valuable tool for investigating AP-3 complex assembly, cargo interactions, and pathogenic mutations. Its applications span in vitro binding assays, structural studies, and functional rescue experiments in AP3S2-deficient models. By enabling precise analysis of AP3S2's molecular interactions, recombinant protein technology advances our understanding of vesicular trafficking mechanisms and their implications in rare genetic diseases and neurological conditions.
×
