| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKK3 |
| Uniprot No | Q9UBP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-350aa |
| 氨基酸序列 | APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEE MEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNN QTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPC RGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCA FQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGL LCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQEL EDLERSLTEEMALGEPAAAAAALLGGEEI |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与DKK3重组蛋白相关的文献摘要概括：
1. **"Recombinant DKK3 attenuates renal fibrosis via modulating Wnt/β-catenin signaling"**
- 作者：Zhou et al.
- 摘要：研究发现重组DKK3蛋白通过抑制Wnt/β-catenin通路，减少肾间质纤维化模型中胶原沉积和成纤维细胞活化，提示其潜在抗纤维化治疗价值。
2. **"DKK3 overexpression inhibits endothelial-mesenchymal transition in cardiac fibrosis through modulation of Wnt signaling"**
- 作者：Li et al.
- 摘要：利用重组DKK3蛋白干预心肌纤维化模型，发现其通过调控Wnt信号通路抑制内皮-间质转化(EndMT)，改善心脏功能并减少纤维化标志物表达。
3. **"Recombinant DKK3 protein suppresses tumor growth by antagonizing cancer-associated fibroblast activation in pancreatic cancer"**
- 作者：Wang et al.
- 摘要：实验证明重组DKK3可抑制胰腺癌微环境中癌症相关成纤维细胞(CAFs)的活化，降低肿瘤侵袭性并增强化疗敏感性，机制涉及TGF-β和Wnt通路交互作用。
4. **"Production and functional characterization of bioactive recombinant human DKK3 in a mammalian expression system"**
- 作者：Zhang et al.
- 摘要：该研究优化了人源DKK3重组蛋白在哺乳动物细胞中的表达与纯化流程，验证其生物活性(如抑制Wnt3a诱导的细胞反应)，为后续研究提供标准化蛋白制备方案。
注：以上内容基于公开文献概括，实际文献需通过PubMed或Web of Science检索标题确认。
DKK3 (Dickkopf-related protein 3) is a secreted glycoprotein belonging to the Dickkopf family, known for its regulatory role in the Wnt signaling pathway. Unlike other DKK members (e.g., DKK1) that primarily inhibit canonical Wnt/β-catenin signaling, DKK3 exhibits a more complex, context-dependent interaction with Wnt and other pathways, including TGF-β and PI3K/AKT. Structurally, it contains two conserved cysteine-rich domains (CRD1 and CRD2) stabilized by disulfide bonds, critical for its stability and receptor binding.
DKK3 is broadly expressed in embryonic tissues and certain adult organs (e.g., kidneys, heart, and blood vessels), playing roles in tissue homeostasis, fibrosis regulation, and tumor suppression. It is implicated in modulating cell proliferation, apoptosis, and differentiation. In cancer, DKK3 often acts as a tumor suppressor, with its downregulation linked to tumor progression via epigenetic silencing or promoter hypermethylation. Conversely, elevated DKK3 in fibrotic diseases (e.g., liver or kidney fibrosis) suggests a pro-fibrotic role, highlighting its dual regulatory functions.
Recombinant DKK3 protein, produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications, serves as a key tool for studying DKK3-mediated signaling mechanisms. Its therapeutic potential is being explored in regenerative medicine, anti-fibrotic therapies, and cancer treatment. Challenges in production include maintaining structural integrity and bioactivity, necessitating stringent quality control. Recent studies also investigate its utility as a biomarker for diseases like chronic kidney disease and certain cancers, underscoring its clinical relevance.
×
