| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELMO1 |
| Uniprot No | Q92556 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-727aa |
| 氨基酸序列 | MPPPADIVKVAIEWPGAYPKLMEIDQKKPLSAIIKEVCDGWSLANHEYFA LQHADSSNFYITEKNRNEIKNGTILRLTTSPAQNAQQLHERIQSSSMDAK LEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERYQKLQKIMKPC FGDMLSFTLTAFVELMDHGIVSWDTFSVAFIKKIASFVNKSAIDISILQR SLAILESMVLNSHDLYQKVAQEITIGQLIPHLQGSDQEIQTYTIAVINAL FLKAPDERRQEMANILAQKQLRSIILTHVIRAQRAINNEMAHQLYVLQVL TFNLLEDRMMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKS MYTRDYKKLGFINHVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVL ENSSREDKHECPFGRSSIELTKMLCEILKVGELPSETCNDFHPMFFTHDR SFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMRALTTKPSSLD QFKSKLQNLSYTEILKIRQSERMNQEDFQSRPILELKEKIQPEILELIKQ QRLNRLVEGTCFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVP HDSLQDKLPVADIKAVVTGKDCPHMKEKGALKQNKEVLELAFSILYDSNC QLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLD LENIQIPDAPPPIPKEPSNYDFVYDCN |
| 预测分子量 | 110 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ELMO1重组蛋白的3篇参考文献示例(基于模拟数据，实际文献需通过学术数据库查询确认)：
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1. **文献名称**：*Structural Basis of ELMO1 Regulation by the Autoinhibitory SH3-α9 Helix*
**作者**：Lu, M., et al.
**摘要**：本研究解析了ELMO1蛋白的自抑制结构，通过重组表达纯化人源ELMO1蛋白，结合X射线晶体学发现其SH3结构域与α9螺旋相互作用，调控与Dock180的结合及Rac1激活，为细胞迁移机制提供结构依据。
2. **文献名称**：*ELMO1-Dock180 Complex Promotes Metastatic Invasion in Breast Cancer*
**作者**：Ahmed, A.F., et al.
**摘要**：文章利用重组ELMO1蛋白进行体外侵袭实验，证明ELMO1-Dock180复合体通过激活Rac1信号促进乳腺癌细胞迁移和转移，提示其作为潜在治疗靶点。
3. **文献名称**：*Recombinant ELMO1 Enhances Phagocytosis in Macrophages via Rac1 Signaling*
**作者**：Park, D., et al.
**摘要**：研究通过大肠杆菌表达系统获得功能性重组ELMO1蛋白，发现其与Dock180协同增强巨噬细胞吞噬功能，依赖Rac1通路，为免疫调节研究提供新思路。
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如需具体文献，建议通过**PubMed**或**Google Scholar**搜索关键词“ELMO1 recombinant protein”或结合功能关键词(如“phagocytosis”“cancer metastasis”)获取最新研究。
ELMO1 (Engulfment and Cell Motility Protein 1) is a conserved cytoplasmic protein critical for regulating cell migration, phagocytosis, and cytoskeletal dynamics. It was initially identified in *Caenorhabditis elegans* for its role in apoptotic cell clearance and later found to function across eukaryotes. Structurally, ELMO1 contains a pleckstrin homology (PH) domain and proline-rich motifs (PxxP), enabling interactions with signaling molecules like Dock180 (Dedicator of Cytokinesis 1), forming the ELMO-Dock complex. This complex acts as a guanine nucleotide exchange factor (GEF) to activate Rac GTPases, which drive actin reorganization, membrane protrusion, and cell motility—processes essential for immune responses, tissue development, and cancer metastasis.
Recombinant ELMO1 protein is engineered for experimental studies to dissect its molecular mechanisms and interactions. Produced via heterologous expression systems (e.g., bacterial, mammalian), it retains functional domains for binding partners such as Dock180. Rac, or integrins. Researchers use purified ELMO1 to study its role in signaling pathways (e.g., Rho GTPase regulation), cellular behaviors (e.g., chemotaxis), and disease contexts. For example, ELMO1 dysregulation is linked to cancer progression, inflammatory disorders, and diabetic nephropathy, making the recombinant protein valuable for drug screening or mechanistic studies.
Its applications extend to structural biology (e.g., crystallography to resolve interaction interfaces) and functional assays (e.g., co-immunoprecipitation, migration assays). By leveraging recombinant ELMO1. scientists aim to uncover therapeutic targets for diseases driven by aberrant cell motility or immune dysfunction.
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