| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P2RX1 |
| Uniprot No | P51575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MARRFQEELAAFLFEYDTPRMVLVRNKKVGVIFRLIQLVVLVYVIGWVFLYEKGYQTSSGLISSVSVKLKGLAVTQLPGLGPQVWDVADYVFPAQGDNSFVVMTNFIVTPKQTQGYCAEHPEGGICKEDSGCTPGKAKRKAQGIRTGKCVAFNDTVKTCEIFGWCPVEVDDDIPRPALLREAENFTLFIKNSISFPRFKVNRRNLVEEVNAAHMKTCLFHKTLHPLCPVFQLGYVVQESGQNFSTLAEKGGVVGITIDWHCDLDWHVRHCRPIYEFHGLYEEKNLSPGFNFRFARHFVENGTNYRHLFKVFGIRFDILVDGKAGKFDIIPTMTTIGSGIGIFGVATVLCDLLLLHILPKRHYYKQKKFKYAEDMGPGAAERDLAATSSTLGLQENMRTS |
| 预测分子量 | 44,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于P2RX1重组蛋白的3篇代表性文献摘要：
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1. **标题**: "Pharmacological characterization of recombinant human P2X1 receptor expressed in HEK293 cells"
**作者**: Valente et al. (2016)
**摘要**: 本研究在HEK293细胞中成功表达了重组人源P2RX1蛋白，并通过膜片钳技术分析了其药理学特性。结果显示，ATP及其类似物(如α,β-meATP)可有效激活受体，但脱敏速度较快，揭示了P2RX1在快速信号传导中的潜在作用。
2. **标题**: "Cloning and functional expression of a rat P2X1 receptor from rat vas deferens"
**作者**: Rettinger et al. (2000)
**摘要**: 研究报道了大鼠P2RX1基因的克隆及其在非洲爪蟾卵母细胞中的异源表达。通过电生理学实验证实，重组受体对ATP高度敏感，且突变关键氨基酸残基(如K68)显著降低配体结合能力，揭示了P2RX1的分子功能机制。
3. **标题**: "Characterization of ATP binding to P2X1 receptors using recombinant protein expressed in insect cells"
**作者**: Ennion et al. (1999)
**摘要**: 利用杆状病毒-昆虫细胞系统表达人P2RX1重组蛋白，并通过放射性配体结合实验分析了ATP的结合特性。研究发现，二价阳离子(如Mg²⁺)可调节ATP亲和力，为P2X受体家族的变构调控机制提供了证据。
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**说明**：
- 上述文献聚焦P2RX1重组蛋白的表达系统(如HEK293、昆虫细胞)、功能分析(电生理、配体结合)及结构-功能关系研究。
- 若需具体DOI或期刊信息，建议通过PubMed或Web of Science以标题/作者关键词检索获取全文。
P2RX1. a member of the P2X receptor family, is an ATP-gated ion channel involved in cellular signaling pathways. It is encoded by the P2RX1 gene and functions as a trimeric protein, with each subunit containing two transmembrane domains connected by a large extracellular ligand-binding loop. This receptor is predominantly expressed in neurons, smooth muscle cells, and platelets, where it mediates rapid responses to extracellular ATP released during tissue injury or cellular stress. Upon ATP binding, P2RX1 undergoes conformational changes, allowing cation influx (Na⁺, Ca²⁺) that triggers downstream processes like neurotransmission, muscle contraction, and platelet activation.
Research highlights its role in physiological and pathological conditions. In the cardiovascular system, P2RX1 contributes to thrombus formation by amplifying platelet activation, making it a potential therapeutic target for thrombosis. In the nervous system, it modulates synaptic transmission and pain signaling. Dysregulation of P2RX1 has been linked to chronic pain syndromes, bladder dysfunction, and neurodegenerative disorders.
Recombinant P2RX1 protein, typically produced in heterologous expression systems (e.g., HEK293 cells), enables detailed structural and functional studies. Its purified form is used to investigate receptor-ligand interactions, ion selectivity, and allosteric modulation. Researchers employ techniques like electrophysiology, fluorescence imaging, and cryo-EM to characterize its activation mechanisms. Recombinant P2RX1 also serves as a tool for drug screening, aiding the development of selective agonists/antagonists. However, challenges remain in stabilizing its trimeric conformation and mimicking native membrane environments in vitro. Studies using recombinant P2RX1 continue to advance understanding of purinergic signaling and its therapeutic applications.
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