| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKK2 |
| Uniprot No | Q9UBU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-259aa |
| 氨基酸序列 | SQIGSSRAKL NSIKSSLGGE TPGQAANRSA GMYQGLAFGG SKKGKNLGQA YPCSSDKECE VGRYCHSPHQ GSSACMVCRR KKKRCHRDGM CCPSTRCNNG ICIPVTESIL TPHIPALDGT RHRDRNHGHY SNHDLGWQNL GRPHTKMSHI KGHEGDPCLR SSDCIEGFCC ARHFWTKICK PVLHQGEVCT KQRKKGSHGL EIFQRCDCAK GLSCKVWKDA TYSSKARLHV CQKI |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DKK2重组蛋白的3篇参考文献概括(基于公开研究主题整理，非真实文献，仅供参考格式)：
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1. **文献名称**: *DKK2 inhibits Wnt signaling to regulate osteoblast differentiation*
**作者**: Li, X., et al.
**摘要**: 研究利用重组DKK2蛋白，证明其通过结合LRP5/6受体抑制经典Wnt信号通路，抑制成骨细胞分化，并揭示其在骨代谢疾病中的潜在调控作用。
2. **文献名称**: *Recombinant DKK2 promotes tumor progression via non-canonical Wnt signaling in colorectal cancer*
**作者**: Mao, B., et al.
**摘要**: 通过体外实验发现，重组DKK2蛋白激活非经典Wnt/JNK通路，促进结直肠癌细胞侵袭转移，表明DKK2可能作为癌症治疗的靶点。
3. **文献名称**: *DKK2 recombinant protein enhances cardiomyocyte regeneration by modulating Wnt/β-catenin*
**作者**: Zhang, Y., et al.
**摘要**: 在小鼠模型中，重组DKK2蛋白通过抑制Wnt/β-catenin信号，促进心肌细胞再生，为心血管再生医学提供新策略。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词 **"DKK2 recombinant protein"** 或 **"DKK2 Wnt signaling"**，筛选近五年研究以获取最新进展。
DKK2 (Dickkopf-related protein 2) is a secreted glycoprotein belonging to the Dickkopf family, known for its role in modulating Wnt signaling pathways. It functions as a biphasic regulator, primarily interacting with Wnt co-receptors LRP5/6 and Kremen proteins to either inhibit or activate Wnt/β-catenin signaling depending on cellular context. Structurally, DKK2 contains two conserved cysteine-rich domains critical for receptor binding.
In embryonic development, DKK2 is essential for tissue patterning, particularly in limb formation, neural crest cell migration, and organogenesis. Its expression is tightly regulated, with dysregulation linked to pathological conditions. In cancer, DKK2 exhibits dual roles: it acts as a tumor suppressor by antagonizing Wnt-driven proliferation in some contexts (e.g., colorectal cancer) but promotes metastasis in others (e.g., hepatocellular carcinoma) through non-canonical Wnt pathways.
Recombinant DKK2 protein is produced using mammalian or insect expression systems to ensure proper post-translational modifications. Purified variants often include tags (e.g., His, Fc) for isolation and detection. Researchers employ it to study Wnt signaling dynamics, cellular differentiation, and tumor microenvironment interactions. Emerging therapeutic applications explore DKK2-neutralizing antibodies or agonists for bone disorders, as DKK2 influences osteoblast/osteoclast balance, and for neurodegenerative diseases where Wnt dysregulation occurs. Current challenges include understanding tissue-specific signaling switches and optimizing delivery methods for clinical translation.
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