| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSTa |
| Uniprot No | Q86UW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MEPGRTQIKLDPRYTADLLEVLKTNYGIPSACFSQPPTAAQLLRALGPVE LALTSILTLLALGSIAIFLEDAVYLYKNTLCPIKRRTLLWKSSAPTVVSV LCCFGLWIPRSLVLVEMTITSFYAVCFYLLMLVMVEGFGGKEAVLRTLRD TPMMVHTGPCCCCCPCCPRLLLTRKKLQLLMLGPFQYAFLKITLTLVGLF LVPDGIYDPADISEGSTALWINTFLGVSTLLALWTLGIISRQARLHLGEQ NMGAKFALFQVLLILTALQPSIFSVLANGGQIACSPPYSSKTRSQVMNCH LLILETFLMTVLTRMYYRRKDHKVGYETFSSPDLDLNLKA |
| 预测分子量 | 37,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于OSTa重组蛋白的典型参考文献示例(注：内容为模拟概括，非真实文献)：
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1. **文献名称**: *Functional co-expression of OSTα and OSTβ in human embryonic kidney cells enhances bile acid-dependent transport*
**作者**: Dawson PA, et al.
**摘要**: 研究报道了人源OSTα/OSTβ异源二聚体在HEK293细胞中的重组共表达，证实二者协同增强牛磺胆酸的跨膜转运能力，并揭示了其pH依赖性转运特性，为胆汁酸代谢研究提供模型。
2. **文献名称**: *Molecular characterization of recombinant mouse OSTα-OSTβ complex and its role in cellular sterol transport*
**作者**: Li N, et al.
**摘要**: 通过昆虫杆状病毒系统表达小鼠OSTα-OSTβ复合体，纯化后分析其与胆固醇衍生物的结合能力，发现该复合体通过调控细胞内固醇分布影响脂代谢通路。
3. **文献名称**: *Cryo-EM structure of the human OSTα-OSTβ transporter reveals a novel protein fold*
**作者**: Garcia KC, et al.
**摘要**: 首次解析人源OSTα-OSTβ复合体的冷冻电镜结构(分辨率3.2Å)，发现OSTβ通过跨膜螺旋锚定OSTα形成非典型转运通道，为靶向该蛋白的药物设计提供结构基础。
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**说明**：OSTα(有机溶质转运蛋白α亚基)常与OSTβ形成异源二聚体，研究多聚焦于其在胆汁酸、固醇类物质跨膜转运中的功能机制及疾病关联。实际文献可通过PubMed等数据库以关键词"OSTα recombinant"或"OSTα-OSTβ complex"检索。
OSTa (Organic Solute Transporter alpha) is a membrane protein that plays a critical role in the transport of organic solutes, particularly bile acids and steroid derivatives, across cellular membranes. It functions as a heterodimer with its partner protein OSTβ, forming a functional transporter complex primarily expressed in the liver, intestine, and kidney. This transporter system facilitates the efflux of substrates from cells to extracellular fluids or blood, contributing to bile acid homeostasis, cholesterol metabolism, and detoxification processes. Dysregulation of OSTa/OSTβ has been linked to cholestatic liver diseases, metabolic disorders, and drug resistance in cancer therapy.
Recombinant OSTa protein is produced using biotechnological platforms such as Escherichia coli, insect cells, or mammalian expression systems. Its production enables structural and functional studies to elucidate substrate specificity, transport mechanisms, and interactions with therapeutic agents. Researchers often co-express OSTa with OSTβ to maintain native conformation and activity, as the beta subunit stabilizes OSTa’s membrane localization and enhances substrate binding. Purification typically involves affinity chromatography combined with detergents to solubilize the membrane protein while preserving its functionality.
OSTa’s recombinant form has become vital for drug discovery, particularly in screening compounds that modulate bile acid transport or mitigate cholestasis. It also serves as a tool to investigate drug-drug interactions and pharmacokinetics of bile acid-based therapeutics. Challenges in OSTa recombinant production include maintaining post-translational modifications in heterologous systems and ensuring proper membrane integration. Recent advances in cryo-EM and nanodisc technologies have improved structural resolution, offering insights into its transport cycle and potential therapeutic targeting strategies.
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