纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CTNNd1 |
Uniprot No | O60716 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-968aa |
氨基酸序列 | MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI |
预测分子量 | 108,1 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTNNB1(β-catenin)重组蛋白的3篇参考文献及其摘要概括:
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1. **文献名称**: "Recombinant β-catenin protein production and its crystallographic analysis"
**作者**: Graham et al.
**摘要**: 报道了人源CTNNB1重组蛋白在大肠杆菌中的高效表达与纯化,通过X射线晶体学解析其三维结构,揭示了与E-cadherin结合的关键结构域,为研究Wnt信号通路提供结构基础。
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2. **文献名称**: "Functional characterization of recombinant β-catenin in Wnt signaling activation"
**作者**: Kim et al.
**摘要**: 利用昆虫细胞系统表达CTNNB1重组蛋白,验证其与TCF/LEF转录因子的相互作用,证明重组蛋白可恢复Wnt缺陷细胞的信号传导活性,为靶向药物筛选提供工具。
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3. **文献名称**: "Purification and application of recombinant β-catenin in colorectal cancer models"
**作者**: Martinez et al.
**摘要**: 开发了基于HEK293细胞的CTNNB1重组蛋白制备工艺,证明其在体外可增强结肠癌细胞侵袭能力,并用于筛选抑制β-catenin/APC相互作用的抗癌化合物。
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*注:以上文献信息为示例性质,实际引用时需核实具体文献来源及细节。建议通过PubMed或Web of Science以“CTNNB1 recombinant protein”为关键词检索最新研究。*
**Background of CTNND1 Recombinant Protein**
CTNND1 (Catenin Delta 1), also known as δ-catenin, is a member of the armadillo repeat-containing protein family, primarily recognized for its role in cell-cell adhesion and signaling. It interacts with classical cadherins, such as E-cadherin, and modulates the stability and function of adherens junctions, which are critical for maintaining tissue architecture and cellular polarity. CTNND1 is uniquely expressed in the nervous system and plays a pivotal role in neurodevelopment, synaptic plasticity, and neuronal migration. Dysregulation of CTNND1 has been implicated in neurodegenerative disorders, including Alzheimer’s disease, and certain cancers, where its overexpression is linked to enhanced tumor invasion and metastasis.
Recombinant CTNND1 protein is engineered using expression systems like *E. coli* or mammalian cells to produce high-purity, functional protein for research applications. The recombinant form typically includes affinity tags (e.g., His-tag) for simplified purification and detection. It serves as a vital tool for studying molecular mechanisms underlying cell adhesion, Wnt signaling pathways, and neuronal development. Researchers utilize CTNND1 recombinant protein in *in vitro* binding assays, structural studies, and screening for therapeutic agents targeting CTNND1-associated diseases. Its application also extends to elucidating the role of δ-catenin in cancer progression, particularly in epithelial-mesenchymal transition (EMT) and drug resistance.
Despite its significance, CTNND1's dual role as both a tumor suppressor and promoter in context-dependent scenarios underscores the complexity of its biological functions. Ongoing studies aim to clarify its regulatory networks and therapeutic potential, making recombinant CTNND1 a valuable resource in biomedical research.
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