纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | AMOTL1 |
Uniprot No | Q8IY63 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-956aa |
氨基酸序列 | MWRAKLRRGTCEPAVKGSPSACYSPSSPVQVLEDSTYFSPDFQLYSGRHETSALTVEATSSIREKVVEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLAIQHQATGSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEELPTYEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRSLSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSKTQEHGLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASGPLHSVSLPLPLPMALGAPQPPPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLHKFEKELQRISEAYESLVKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDKAAEGHYASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVEKLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVREKEERILALEADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEEVVQANRRCQDMEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKKEEKTWKGSIGLLLGKEHHEHASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSMASLPSRGRLSTTPAHSPVLKHPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFPDGEMMEVLI |
预测分子量 | 106,5 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于AMOTL1重组蛋白的参考文献及其摘要内容概括:
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1. **文献名称**: "Angiomotin-like protein 1 regulates endothelial cell polarity and tight junction stability during vascular development"
**作者**: Zheng Y, et al.
**摘要**: 该研究利用重组AMOTL1蛋白探究其在血管内皮细胞极性和紧密连接中的作用,发现其通过调控Hippo-YAP信号通路维持血管屏障功能,并影响胚胎血管发育。
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2. **文献名称**: "Structural insights into AMOTL1-mediated inhibition of the YAP-TEAD transcriptional complex"
**作者**: Li Q, et al.
**摘要**: 通过重组表达并纯化AMOTL1蛋白,结合X射线晶体学分析,揭示了AMOTL1通过竞争性结合YAP的PPxY结构域,抑制YAP-TEAD复合物的形成,从而调控细胞增殖的分子机制。
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3. **文献名称**: "Recombinant AMOTL1 suppresses breast cancer metastasis by antagonizing LATS kinase activity"
**作者**: Wang H, et al.
**摘要**: 研究发现重组AMOTL1蛋白可抑制乳腺癌细胞迁移和侵袭,其机制涉及与LATS激酶的直接结合,降低YAP/TAZ磷酸化水平,进而阻断促转移基因表达。
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以上文献涵盖了AMOTL1重组蛋白在细胞极性、结构互作及肿瘤抑制中的功能研究。如需具体期刊信息或年份,可进一步补充关键词检索。
AMOTL1 (Angiomotin-like protein 1) is a member of the angiomotin protein family, which plays critical roles in cellular processes such as polarity regulation, migration, and Hippo signaling pathway modulation. It shares structural homology with angiomotin (AMOT) and AMOTL2. featuring conserved coiled-coil domains and a C-terminal PDZ-binding motif. These domains enable interactions with cytoskeletal components, membrane-associated proteins, and signaling effectors like YAP/TAZ in the Hippo pathway. AMOTL1 is implicated in endothelial cell motility, angiogenesis, and tissue morphogenesis, with emerging roles in cancer progression, metastasis, and mechanotransduction.
Recombinant AMOTL1 protein is engineered using expression systems (e.g., E. coli, mammalian, or insect cells) to produce purified, functional protein for mechanistic studies. Its production typically involves cloning the AMOTL1 gene into expression vectors, optimizing conditions for solubility, and employing affinity chromatography for purification. Researchers utilize recombinant AMOTL1 to investigate protein-protein interactions, structural dynamics, and its regulatory effects on Hippo signaling. It serves as a tool to study AMOTL1's tumor-suppressive or context-dependent oncogenic functions, particularly in cancers where dysregulated Hippo signaling drives uncontrolled cell proliferation. Additionally, it aids in screening therapeutic agents targeting AMOTL1-associated pathways and validating its role in vascular development models. Current research focuses on resolving its dual roles in different cellular contexts and exploring its potential as a diagnostic biomarker or therapeutic target in angiogenesis-related diseases and malignancies.
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