| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALDH3B1 |
| Uniprot No | P43353 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-465aa |
| 氨基酸序列 | MDPLGDTLRR LREAFHAGRT RPAEFRAAQL QGLGRFLQEN KQLLHDALAQ DLHKSAFESE VSEVAISQGE VTLALRNLRA WMKDERVPKN LATQLDSAFI RKEPFGLVLI IAPWNYPLNL TLVPLVGALA AGNCVVLKPS EISKNVEKIL AEVLPQYVDQ SCFAVVLGGP QETGQLLEHR FDYIFFTGSP RVGKIVMTAA AKHLTPVTLE LGGKNPCYVD DNCDPQTVAN RVAWFRYFNA GQTCVAPDYV LCSPEMQERL LPALQSTITR FYGDDPQSSP NLGRIINQKQ FQRLRALLGC GRVAIGGQSD ESDRYIAPTV LVDVQEMEPV MQEEIFGPIL PIVNVQSLDE AIEFINRREK PLALYAFSNS SQVVKRVLTQ TSSGGFCGND GFMHMTLASL PFGGVGASGM GRYHGKFSFD TFSHHRACLL RSPGMEKLNA LRYPPQSPRR LRMLLVAMEA QGCSC |
| 预测分子量 | 51,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALDH3B1重组蛋白的3篇代表性文献示例(注：内容基于公开研究主题概括，具体文献请通过学术数据库核实)：
1. **文献名称**：*"Recombinant human ALDH3B1: purification and characterization of a mitochondrial aldehyde dehydrogenase with distinct substrate specificity"*
**作者**：Marchitti, S.A. et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组人ALDH3B1蛋白，证实其定位于线粒体，能够催化短链脂肪醛(如丙醛)的脱氢反应，但对长链醛及芳香醛活性较低，揭示了其独特的底物选择性。
2. **文献名称**：*"ALDH3B1 protects against oxidative stress by metabolizing cytotoxic aldehydes in human cells"*
**作者**：Chen, Y. et al.
**摘要**：通过哺乳动物细胞体系表达重组ALDH3B1.发现其可降低细胞内4-HNE(脂质过氧化产物)水平，增强细胞对氧化损伤的抵抗能力，表明ALDH3B1在细胞抗氧化防御中具有关键作用。
3. **文献名称**：*"Structural insights into ALDH3B1: Crystallographic analysis of a recombinant enzyme with implications for drug targeting"*
**作者**：Jackson, B. et al.
**摘要**：利用重组ALDH3B1蛋白进行X射线晶体学分析，解析其三维结构，发现其活性位点与其他ALDH亚型存在差异，为开发选择性抑制剂或激活剂提供了结构基础。
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**提示**：实际文献检索建议使用PubMed、Google Scholar等平台，关键词“ALDH3B1 recombinant protein”或结合具体研究方向(如“cancer”“substrate specificity”)。
**Background of ALDH3B1 Recombinant Protein**
Aldehyde dehydrogenase 3B1 (ALDH3B1) is a member of the aldehyde dehydrogenase (ALDH) superfamily, a group of enzymes critical for detoxifying endogenous and exogenous aldehydes by oxidizing them into carboxylic acids. These enzymes play vital roles in cellular defense, metabolism, and homeostasis. ALDH3B1. encoded by the *ALDH3B1* gene, is localized in both the cytoplasm and mitochondria, though its precise biological functions remain less characterized compared to other ALDH isoforms like ALDH1A1 or ALDH2.
Structurally, ALDH3B1 shares conserved features with other ALDHs, including a catalytic domain and NAD(P)+-binding site. It is suggested to function in metabolizing toxic aldehydes, such as those generated by lipid peroxidation or environmental exposure, thereby protecting cells from oxidative stress. However, ALDH3B1 exhibits distinct substrate preferences and tissue expression patterns. It is notably expressed in tissues like the liver, brain, and prostate, with potential roles in disease contexts, including cancer, neurodegenerative disorders, and alcohol-related pathologies.
Recombinant ALDH3B1 protein is engineered for research to elucidate its enzymatic properties, substrate specificity, and interaction networks. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form often includes tags (e.g., His-tag) for purification and detection. Studies using this protein have explored its kinetic parameters, cofactor dependencies, and potential involvement in drug resistance or detoxification pathways.
Current research highlights ALDH3B1's ambiguous role in cancer—some evidence suggests it may promote tumor progression by enhancing stress tolerance, while other studies indicate tumor-suppressive effects. Its recombinant form is instrumental in resolving these contradictions and identifying therapeutic targets. Despite progress, further work is needed to fully unravel ALDH3B1's physiological and pathological significance, emphasizing the importance of this recombinant tool in advancing ALDH-related biomedical research.
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