| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FRS3 |
| Uniprot No | O43559 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-492aa |
| 氨基酸序列 | GSCCSCLNR DSVPDNHPTK FKVTNVDDEG VELGSGVMEL TQSELVLHLH RREAVRWPYL CLRRYGYDSN LFSFESGRRC QTGQGIFAFK CSRAEEIFNL LQDLMQCNSI NVMEEPVIIT RNSHPAELDL PRAPQPPNAL GYTVSSFSNG CPGEGPRFSA PRRLSTSSLR HPSLGEESTH ALIAPDEQSH TYVNTPASED DHRRGRHCLQ PLPEGQAPFL PQARGPDQRD PQVFLQPGQV KFVLGPTPAR RHMVKCQGLC PSLHDPPHHN NNNEAPSECP AQPKCTYENV TGGLWRGAGW RLSPEEPGWN GLAHRRAALL HYENLPPLPP VWESQAQQLG GEAGDDGDSR DGLTPSSNGF PDGEEDETPL QKPTSTRAAI RSHGSFPVPL TRRRGSPRVF NFDFRRPGPE PPRQLNYIQV ELKGWGGDRP KGPQNPSSPQ APMPTTHPAR SSDSYAVIDL KKTVAMSNLQ RALPRDDGTA RKTRHNSTDL PL |
| 预测分子量 | 54,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FRS3重组蛋白的假设性参考文献示例，基于该领域常见研究方向总结，供参考：
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1. **文献名称**: *Structural and Functional Analysis of FRS3 in FGFR Signaling*
**作者**: Smith A, et al.
**摘要**: 研究通过重组表达FRS3蛋白，解析其与FGFR1的相互作用结构域，并证实其在MAPK信号通路激活中的支架作用，为靶向FRS3的癌症治疗提供依据。
2. **文献名称**: *Recombinant FRS3 Production in E. coli and Phosphorylation Dynamics*
**作者**: Lee J, et al.
**摘要**: 报道利用大肠杆菌系统高效表达可溶性FRS3重组蛋白，并通过体外实验验证其酪氨酸磷酸化对Grb2结合的依赖性。
3. **文献名称**: *FRS3 Knockdown and Rescue with Recombinant Protein in Neuronal Development*
**作者**: Chen R, et al.
**摘要**: 使用重组FRS3蛋白回补实验，证明其在神经祖细胞分化中的必要性，并揭示其通过PI3K-Akt通路调控细胞迁移的机制。
4. **文献名称**: *Cryo-EM Structure of the FRS3-FGFR1 Complex*
**作者**: Müller T, et al.
**摘要**: 通过冷冻电镜解析FRS3重组蛋白与FGFR1的复合体结构，阐明磷酸化位点对下游信号分子招募的空间调控模式。
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**注意**：以上文献为示例性内容，实际引用需查询PubMed/Google Scholar等数据库获取真实文献。建议使用关键词“FRS3 recombinant protein”“FGF signaling”“scaffold protein”进行检索。
**Background of FRS3 Recombinant Protein**
FRS3 (Fibroblast Growth Factor Receptor Substrate 3), also known as FRS2β or SNT-2. is an adaptor protein involved in intracellular signaling pathways triggered by fibroblast growth factor receptors (FGFRs) and neurotrophin receptors (e.g., TrkA). It was initially identified as a homolog of FRS2 (FRS2α), sharing structural and functional similarities. FRS3 contains an N-terminal myristoylation site for membrane anchoring, a phosphotyrosine-binding (PTB) domain for receptor interaction, and multiple tyrosine phosphorylation sites that recruit downstream signaling molecules like GRB2 and SOS1. activating MAPK/ERK and PI3K-AKT pathways.
Recombinant FRS3 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research applications. Its production enables the study of FRS3's role in cellular processes such as proliferation, differentiation, and survival. Dysregulation of FRS3 has been linked to developmental disorders and cancers, particularly in contexts where FGFR signaling is aberrant. Researchers utilize recombinant FRS3 to investigate its interactions with signaling complexes, post-translational modifications, and potential therapeutic targeting. Its structural and functional conservation across species also makes it valuable for translational studies in model organisms. Overall, FRS3 recombinant protein serves as a critical tool for dissecting signaling networks and developing strategies to modulate pathological signaling cascades.
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