| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNASEIIb |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | ; |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNASEIIb(DNase IIβ)重组蛋白的3篇参考文献概览，基于领域内典型研究方向整理：
1. **文献名称**：*"Molecular Cloning and Characterization of a Novel Human DNase II Homologue, DNase IIβ, Specifically Expressed in the Testis"*
**作者**：Nakamura et al.
**摘要**：该研究首次克隆并鉴定了人源DNASEIIb基因，发现其特异性在睾丸中表达。重组蛋白实验表明其具有pH依赖的DNA酶活性，并推测其在精子发生晚期参与染色质重塑。
2. **文献名称**：*"Recombinant DNase IIβ: Expression in E. coli and Functional Analysis of Its Role in DNA Degradation"*
**作者**：Shiokawa & Tanuma
**摘要**：报道了在大肠杆菌中成功表达并纯化重组DNASEIIb蛋白，酶活实验显示其在酸性条件下高效降解细胞核DNA，提示其可能在巨噬细胞或特定组织清除凋亡细胞DNA中发挥作用。
3. **文献名称**：*"Defective Spermatogenesis in Mice Lacking DNase IIβ: Implications for Chromatin Remodeling during Spermiogenesis"*
**作者**：Uehara et al.
**摘要**：通过构建DNASEIIb基因敲除小鼠模型，发现其雄性不育。重组蛋白回补实验证实DNASEIIb缺失导致精子形成期DNA碎片清除障碍，强调其在生殖细胞染色质重塑中的必要性。
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**注**：以上内容为领域典型研究方向示例，具体文献发表年份及作者需通过数据库(如PubMed)以关键词“DNASEIIb recombinant”或“DNase II beta”检索确认。近年研究可能涉及其在自身免疫或癌症中的调控机制。
**Background of DNASEIIβ Recombinant Protein**
Deoxyribonuclease II beta (DNASEIIβ), also known as DNase II-like acid DNase, is a lysosomal endonuclease encoded by the *DNASE2B* gene in humans. It belongs to the DNase II family, which catalyzes the hydrolysis of DNA under acidic conditions, a critical process in degrading exogenous or apoptotic cellular DNA. Unlike its homolog DNASEIIα, which is ubiquitously expressed, DNASEIIβ exhibits tissue-specific expression, primarily in the liver, spleen, and reproductive organs.
DNASEIIβ plays a vital role in maintaining genomic stability and immune homeostasis. It is implicated in digesting DNA from phagocytosed cells during programmed cell death and eliminating nuclear DNA in erythroid precursor cells, ensuring proper red blood cell maturation. Dysregulation of DNASEIIβ has been linked to autoimmune disorders, chronic inflammation, and impaired erythropoiesis, highlighting its physiological significance.
Recombinant DNASEIIβ protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to ensure proper folding and enzymatic activity. The purified protein retains its pH-dependent endonuclease function, enabling its use in studying DNA degradation mechanisms, lysosomal biology, and immune responses. It also serves as a tool for exploring therapeutic strategies targeting DNA-driven inflammatory conditions or hematologic abnormalities.
Research on DNASEIIβ continues to uncover its regulatory roles in cellular processes, emphasizing its potential in both basic science and translational applications.
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