| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAAM2 |
| Uniprot No | Q86T65 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1068aa |
| 氨基酸序列 | MAPRKRSHHGLGFLCCFGGSDIPEINLRDNHPLQFMEFSSPIPNAEELNIRFAELVDELDLTDKNREAMFALPPEKKWQIYCSKKKEQEDPNKLATSWPDYYIDRINSMAAMQSLYAFDEEETEMRNQVVEDLKTALRTQPMRFVTRFIELEGLTCLLNFLRSMDHATCESRIHTSLIGCIKALMNNSQGRAHVLAQPEAISTIAQSLRTENSKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQVYAAERTRFQTLLNELDRSLGRYRDEVNLKTAIMSFINAVLNAGAGEDNLEFRLHLRYEFLMLGIQPVIDKLRQHENAILDKHLDFFEMVRNEDDLELARRFDMVHIDTKSASQMFELIHKKLKYTEAYPCLLSVLHHCLQMPYKRNGGYFQQWQLLDRILQQIVLQDERGVDPDLAPLENFNVKNIVNMLINENEVKQWRDQAEKFRKEHMELVSRLERKERECETKTLEKEEMMRTLNKMKDKLARESQELRQARGQVAELVAQLSELSTGPVSSPPPPGGPLTLSSSMTTNDLPPPPPPLPFACCPPPPPPPLPPGGPPTPPGAPPCLGMGLPLPQDPYPSSDVPLRKKRVPQPSHPLKSFNWVKLNEERVPGTVWNEIDDMQVFRILDLEDFEKMFSAYQRHQKELGSTEDIYLASRKVKELSVIDGRRAQNCIILLSKLKLSNEEIRQAILKMDEQEDLAKDMLEQLLKFIPEKSDIDLLEEHKHEIERMARADRFLYEMSRIDHYQQRLQALFFKKKFQERLAEAKPKVEAILLASRELVRSKRLRQMLEVILAIGNFMNKGQRGGAYGFRVASLNKIADTKSSIDRNISLLHYLIMILEKHFPDILNMPSELQHLPEAAKVNLAELEKEVGNLRRGLRAVEVELEYQRRQVREPSDKFVPVMSDFITVSSFSFSELEDQLNEARDKFAKALMHFGEHDSKMQPDEFFGIFDTFLQAFSEARQDLEAMRRRKEEEERRARMEAMLKEQRERERWQRQRKVLAAGSSLEEGGEFDDLVSALRSGEVFDKDLCKLKRSRKRSGSQALEVTRERAINRLNY |
| 预测分子量 | 123 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DAAM2重组蛋白的3篇示例参考文献(注：部分内容为虚构示例，实际文献需通过学术数据库检索确认)：
---
1. **标题**: "Expression and Functional Analysis of DAAM2 Recombinant Protein in Wnt/PCP Signaling"
**作者**: Smith J, et al.
**摘要**: 本研究通过昆虫细胞表达系统制备了DAAM2重组蛋白，并验证其与Dishevelled蛋白的体外结合能力。实验表明，DAAM2通过formin结构域促进微丝聚合，并增强Wnt/PCP通路介导的细胞极性调控。
2. **标题**: "DAAM2 Recombinant Protein Facilitates Axon Guidance via Actin Remodeling"
**作者**: Lee S, et al.
**摘要**: 作者利用大肠杆菌系统表达并纯化DAAM2重组蛋白，发现其能显著诱导神经细胞中actin纤维的重排。进一步实验证明，DAAM2通过激活RhoA信号通路调控神经元突触的形态发生。
3. **标题**: "Structural Characterization of DAAM2 FH1-FH2 Domains Using Recombinant Protein"
**作者**: Zhang Y, et al.
**摘要**: 通过体外重组表达DAAM2的FH1-FH2功能域，结合冷冻电镜技术解析其三维结构，揭示了该区域在actin成核中的关键作用，为DAAM2相关疾病的机制研究提供结构基础。
---
如需真实文献，建议在PubMed、Web of Science等平台以“DAAM2 recombinant protein”或“DAAM2 purification”为关键词检索近年研究。
DAAM2 (Dishevelled-Associated Activator of Morphogenesis 2) is a cytoskeletal regulatory protein belonging to the Formin family, known for its role in actin polymerization and microtubule dynamics. It is a downstream effector of the Wnt/planar cell polarity (PCP) signaling pathway, which governs tissue patterning, cell migration, and polarized morphogenesis during embryonic development. DAAM2 interacts with Dishevelled (Dvl) proteins, bridging Wnt signals to Rho GTPase activation, thereby modulating cytoskeletal rearrangements critical for processes like neural tube closure, axon guidance, and organogenesis.
Structurally, DAAM2 contains Formin homology 1/2 (FH1/FH2) domains essential for actin assembly, a GTPase-binding domain (GBD), and a Dishevelled-binding interface. Unlike its homolog DAAM1. DAAM2 exhibits distinct expression patterns, predominantly in the central nervous system, and plays specialized roles in neurodevelopment. Studies link DAAM2 dysfunction to neurodevelopmental disorders, cancer metastasis, and ciliopathies, highlighting its biomedical relevance.
Recombinant DAAM2 proteins are engineered for in vitro studies to dissect molecular mechanisms. Typically produced in bacterial or mammalian expression systems with affinity tags (e.g., His-tag), these purified proteins enable biochemical assays, structural analyses, and interaction mapping. Recent research leverages DAAM2 recombinant proteins to explore its crosstalk with other polarity pathways (e.g., Notch, Hippo) and its potential as a therapeutic target in neural injury or tumor microenvironments. Its dual regulation of actin and microtubules also positions it as a key node in studying cytoskeletal coordination during cell polarization and tissue remodeling.
×
