| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AOC2 |
| Uniprot No | O75106 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-756aa |
| 氨基酸序列 | QPPHCPSVSHRAQPWPHPGQSQLFADLSREELTAVMRFLTQRLGPGLVDAAQAQPSDNCIFSVELQLPPKAAALAHLDRGSPPPAREALAIVLFGGQPQPNVSELVVGPLPHPSYMRDVTVERHGGPLPYHRRPVLRAEFTQMWRHLKEVELPKAPIFLSSTFNYNGSTLAAVHATPRGLRSGDRATWMALYHNISGVGLFLHPVGLELLLDHRALDPAHWTVQQVFYLGHYYADLGQLEREFKSGRLEVVRVPLPPPNGASSLRSRNSPGPLPPLQFSPQGSQYSVQGNLVVSSLWSFTFGHGVFSGLRIFDVRFQGERIAYEVSVQECVSIYGADSPKTMLTRYLDSSFGLGRNSRGLVRGVDCPYQATMVDIHILVGKGAVQLLPGAVCVFEEAQGLPLRRHHNYLQNHFYGGLASSALVVRSVSSVGNYDYIWDFVLYPNGALEGRVHATGYINTAFLKGGEEGLLFGNRVGERVLGTVHTHAFHFKLDLDVAGLKNWVVAEDVVFKPVAAPWNPEHWLQRPQLTRQVLGKEDLTAFSLGSPLPRYLYLASNQTNAWGHQRGYRIQIHSPLGIHIPLESDMERALSWGRYQLVVTQRKEEESQSSSIYHQNDIWTPTVTFADFINNETLLGEDLVAWVTASFLHIPHAEDIPNTVTLGNRVGFLLRPYNFFDEDPSIFSPGSVYFEKGQDAGLCSINPVACLPDLAACVPDLPPFSYHGF |
| 预测分子量 | 85.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与AOC2重组蛋白相关的模拟参考文献(注：内容为学术场景虚构，实际文献需通过专业数据库检索)：
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1. **文献名称**: *Structural characterization of recombinant human AOC2 and its role in polyamine metabolism*
**作者**: Smith, J. et al.
**摘要**: 本研究通过大肠杆菌系统表达并纯化AOC2重组蛋白，解析其X射线晶体结构(分辨率2.1Å)，揭示其铜离子结合位点和底物识别机制，证实其在多胺代谢中的氧化酶活性。
2. **文献名称**: *AOC2 overexpression inhibits tumor angiogenesis via ROS-mediated endothelial cell apoptosis*
**作者**: Li, X. & Wang, Y.
**摘要**: 利用CHO细胞表达AOC2重组蛋白，发现其通过产生活性氧(ROS)诱导血管内皮细胞凋亡，显著抑制小鼠移植瘤模型的血管生成，提示AOC2作为抗肿瘤靶点的潜力。
3. **文献名称**: *Optimization of AOC2 recombinant production in Pichia pastoris for industrial-scale applications*
**作者**: Gonzalez, R. et al.
**摘要**: 通过优化毕赤酵母表达系统的启动子、培养条件及糖基化修饰，将AOC2重组蛋白产量提升至2.8 g/L，为工业化生产高活性AOC2酶制剂提供技术方案。
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如需真实文献，建议在PubMed或Web of Science中检索关键词 "AOC2 recombinant protein" 或 "recombinant amine oxidase copper-containing 2"。
**Background of AOC2 Recombinant Protein**
AOC2 (Amine Oxidase, Copper-Dependent 2), also known as lysyl oxidase homolog 2 (LOXL2), is a member of the lysyl oxidase family of enzymes that play critical roles in extracellular matrix (ECM) remodeling and tissue homeostasis. These copper-dependent oxidases catalyze the cross-linking of collagen and elastin by oxidizing lysine residues, thereby stabilizing structural proteins and influencing mechanical properties of tissues. AOC2 is particularly implicated in pathological processes such as fibrosis, cancer progression, and metastasis due to its ability to promote ECM stiffness and activate pro-invasive signaling pathways.
Recombinant AOC2 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, bioactive form of the enzyme for research and therapeutic development. Its production often involves optimizing conditions to ensure proper post-translational modifications, including copper incorporation, which is essential for enzymatic activity.
In research, recombinant AOC2 is utilized to study its role in tumor microenvironment modulation, epithelial-mesenchymal transition (EMT), and fibrotic diseases. Inhibitors targeting AOC2 are being explored as potential therapeutics to counteract fibrosis or metastatic cancer. Additionally, recombinant AOC2 serves as a tool for screening drug candidates or elucidating molecular mechanisms underlying ECM dysregulation.
Despite its promise, challenges remain in understanding tissue-specific functions and balancing therapeutic inhibition to avoid off-target effects on normal ECM maintenance. Ongoing studies aim to clarify its dual roles in physiological repair versus pathological progression, highlighting AOC2 as a molecule of significant biomedical interest.
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