| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIMT1 |
| Uniprot No | Q9UNQ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MMPKVKSGAI GRRRGRQEQR RELKSAGGLM FNTGIGQHIL KNPLIINSII DKAALRPTDV VLEVGPGTGN MTVKLLEKAK KVVACELDPR LVAELHKRVQ GTPVASKLQV LVGDVLKTDL PFFDTCVANL PYQISSPFVF KLLLHRPFFR CAILMFQREF ALRLVAKPGD KLYCRLSINT QLLARVDHLM KVGKNNFRPP PKVESSVVRI EPKNPPPPIN FQEWDGLVRI TFVRKNKTLS AAFKSSAVQQ LLEKNYRIHC SVHNIIIPED FSIADKIQQI LTSTGFSDKR ARSMDIDDFI RLLHGFNAEG IHFS |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DIMT1重组蛋白的3篇示例参考文献(内容为模拟虚构，实际文献需通过学术数据库检索)：
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1. **文献名称**: *Structural Insights into DIMT1-Mediated Ribosomal RNA Methylation*
**作者**: Doe, J. et al. (2020)
**摘要**: 本研究解析了DIMT1重组蛋白的晶体结构，揭示了其催化核糖体RNA(rRNA)中保守位点甲基化的分子机制。通过体外重组表达DIMT1蛋白，证实了其对18S rRNA的N6.6'-二甲基腺苷修饰功能，并发现其活性依赖于与核仁蛋白NOP56的相互作用。
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2. **文献名称**: *DIMT1 Regulates Cell Cycle Progression via Ribosome Biogenesis in Cancer Cells*
**作者**: Smith, R. et al. (2018)
**摘要**: 研究利用重组DIMT1蛋白进行功能补偿实验，发现DIMT1缺失导致核糖体组装缺陷，进而阻滞肿瘤细胞G1/S期转换。重组蛋白的过表达可部分恢复核糖体RNA加工，提示DIMT1通过调控rRNA成熟影响细胞增殖。
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3. **文献名称**: *Development of a DIMT1 Knockout Mouse Model Using CRISPR/Cas9*
**作者**: Chen, X. et al. (2022)
**摘要**: 通过重组DIMT1蛋白回补实验，验证了DIMT1基因敲除小鼠胚胎致死表型与rRNA修饰缺陷的直接关联。研究还建立了体外重组DIMT1蛋白活性检测体系，为靶向核糖体RNA修饰的疗法提供工具。
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**备注**：以上文献为示例，实际研究中建议通过PubMed、Google Scholar等平台检索关键词"DIMT1 recombinant protein"或结合具体研究领域(如核糖体生物合成、RNA修饰)筛选文献。
**Background of DIMT1 Recombinant Protein**
DIMT1 (Dim1 Homolog, rRNA Methyltransferase) is a conserved eukaryotic enzyme critical for ribosome biogenesis. It functions as an S-adenosylmethionine-dependent methyltransferase, catalyzing the N6-dimethylation of two adjacent adenine residues (A1850 and A1854 in humans; equivalent to A1832 and A1836 in yeast) in the 18S rRNA of the small ribosomal subunit. This modification occurs during the early stages of ribosome assembly and is essential for proper rRNA processing, subunit maturation, and translational fidelity.
The DIMT1 gene is highly conserved across species, underscoring its fundamental role in cellular function. Studies in yeast and mammalian systems reveal that DIMT1 interacts with ribosome assembly factors and components of the small subunit processome, positioning it as a key regulator of ribosome quality control. Dysregulation of DIMT1 has been linked to developmental defects, cancer, and neurodegenerative disorders, likely due to impaired ribosome function and protein synthesis.
Recombinant DIMT1 protein is typically produced in bacterial (e.g., *E. coli*) or eukaryotic expression systems to preserve its enzymatic activity and structural integrity. Purified DIMT1 is utilized for *in vitro* studies to dissect its methyltransferase mechanism, substrate specificity, and interactions with rRNA or co-factors. It also serves as a tool for drug discovery, particularly in identifying inhibitors that target ribosome biogenesis pathways.
Overall, DIMT1 recombinant protein provides a valuable model for understanding ribosome assembly, epigenetic RNA modifications, and their implications in disease, offering potential therapeutic avenues for conditions linked to translational dysregulation.
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