| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAT1 |
| Uniprot No | P21673 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 5-171aa |
| 氨基酸序列 | VIRPATAADCSDILRLIKELAKYEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMATEE |
| 预测分子量 | 26.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于SAT1重组蛋白的模拟参考文献示例(注：部分信息为示例性概括，实际文献请通过学术数据库检索)：
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1. **文献名称**: *"Cloning and functional characterization of recombinant SAT1: role in polyamine catabolism"*
**作者**: Kramer, D.L., Hacker, H.J., Pegg, A.E.
**摘要**: 本研究通过在大肠杆菌中表达重组SAT1蛋白，验证其亚精胺/精胺N1-乙酰转移酶活性，证明SAT1通过乙酰化多胺调控细胞内多胺稳态，并可能影响细胞增殖和凋亡。
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2. **文献名称**: *"Structural insights into SAT1 enzyme: X-ray crystallography of the recombinant protein"*
**作者**: Wu, H., Wang, Y., & Cole, P.A.
**摘要**: 通过X射线晶体学解析了重组SAT1蛋白的三维结构，揭示了其底物结合位点和催化机制，为开发靶向SAT1的小分子抑制剂提供了结构基础。
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3. **文献名称**: *"SAT1 overexpression in cancer cells: Recombinant protein-based therapeutic implications"*
**作者**: Gupta, S., Kim, S.H., & Tabor, C.W.
**摘要**: 利用重组SAT1蛋白研究其在癌细胞中的过表达效应，发现SAT1通过调节多胺代谢抑制肿瘤生长，提示其作为癌症治疗靶点的潜力。
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建议通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索真实文献，关键词如 *"recombinant SAT1 protein"* 或 *"SAT1 acetyltransferase"*。
**Background of SAT1 Recombinant Protein**
Spermidine/spermine N1-acetyltransferase 1 (SAT1) is a key enzyme in polyamine metabolism, catalyzing the acetylation of spermidine and spermine. This process regulates cellular polyamine levels, which are critical for cell growth, differentiation, and apoptosis. Dysregulation of SAT1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, making it a focal point for therapeutic research.
Recombinant SAT1 protein is engineered using genetic cloning techniques, where the SAT1 gene is inserted into expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce purified, functional protein. This approach ensures high yield, consistency, and avoids contamination from native cellular components. The recombinant protein retains enzymatic activity, enabling researchers to study its structure, substrate specificity, and interactions with inhibitors or modulators.
SAT1’s role in polyamine catabolism links it to oxidative stress responses, as acetylated polyamines are exported or degraded, influencing redox balance. In cancer, SAT1 downregulation is associated with polyamine accumulation and tumor progression, while its upregulation may induce apoptosis. Neurodegenerative studies highlight SAT1’s potential in mitigating toxic polyamine metabolites linked to conditions like Alzheimer’s disease.
As a research tool, recombinant SAT1 aids in drug discovery, particularly for targeting polyamine pathways. It also serves in diagnostic assays to measure enzymatic activity in clinical samples. Ongoing studies explore its therapeutic potential, including SAT1-inducing agents or enzyme replacement strategies. Challenges remain in optimizing stability and delivery for clinical applications, but SAT1 recombinant protein remains a vital resource for understanding polyamine biology and developing targeted therapies.
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