| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WIPF1 |
| Uniprot No | O43516 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTV TNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGL GGLFQAGMPKLRSTANRDNHSGGSRPPLLPPGGRSTSAKPFSPPSGPGRF PVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSLHN RGSPPVPGGPRQPSPGPTPPPPPVRDPPGRSGPLPPPPPVSRNGSTSRAL PATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSP CEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGA PPLPPIPR |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WIPF1重组蛋白的3篇参考文献示例(注：以下内容为示例性概括，实际文献需根据具体研究查询)：
1. **文献名称**：*"Recombinant WIPF1 enhances WASp-mediated actin polymerization in vitro"*
**作者**：Smith A, et al.
**摘要**：研究报道了通过昆虫细胞表达系统成功纯化重组人WIPF1蛋白，并证明其与Wiskott-Aldrich综合征蛋白(WASp)的结合能力。实验显示，WIPF1重组蛋白显著促进WASp依赖的肌动蛋白聚合，为研究细胞骨架调控机制提供了工具。
2. **文献名称**：*"Structural insights into the WIPF1-WASp complex by cryo-EM"*
**作者**：Li J, et al.
**摘要**：该研究利用冷冻电镜解析了重组WIPF1与WASp的复合物结构，揭示了二者相互作用的关键结构域，并发现WIPF1通过特定氨基酸残基稳定WASp的活性构象，为靶向该相互作用的药物设计提供依据。
3. **文献名称**：*"Recombinant WIPF1 rescues immune cell migration defects in WIPF1-deficient mice"*
**作者**：Garcia-Rivera E, et al.
**摘要**：通过在大肠杆菌中表达功能性WIPF1重组蛋白，研究证明其在体外可恢复WIPF1缺陷型T细胞的趋化能力，并改善小鼠模型中免疫细胞的迁移缺陷，提示其潜在治疗应用价值。
(注：如需真实文献，建议通过PubMed或Google Scholar搜索关键词“WIPF1 recombinant protein”或“WIPF1 expression and function”获取具体研究。)
WIPF1 (WAS/WASL-interacting protein family member 1), also known as WASP-interacting protein (WIP), is a cytoskeletal regulatory protein that plays a critical role in actin polymerization and cell motility. It binds to the Wiskott-Aldrich syndrome protein (WASP) and its neural homolog WAVE (WASP-associated protein), stabilizing these key actin-nucleating factors and modulating their activity. This interaction is essential for coordinating actin dynamics, which underlies processes like immune cell migration, phagocytosis, and synaptic formation. Dysregulation of WIPF1 has been linked to immunodeficiency disorders, such as Wiskott-Aldrich syndrome, and cancer metastasis due to its role in invasive cell behavior.
Recombinant WIPF1 protein is produced using expression systems (e.g., E. coli, mammalian cells) to generate purified, functional protein for research and therapeutic applications. It enables mechanistic studies of WIPF1’s structure-function relationships, including its N-terminal WH2 domain for actin binding and C-terminal motifs for WASP/WAVE interactions. Researchers utilize recombinant WIPF1 to investigate signaling pathways involving Rho GTPases, tyrosine kinases, and adaptor proteins that regulate cytoskeletal remodeling. Additionally, it serves as a tool for screening inhibitors targeting WIPF1-WASP complexes in autoimmune or inflammatory diseases. The protein’s stability and bioactivity are optimized through tags (e.g., GST, His-tag) and quality-controlled assays (e.g., co-immunoprecipitation, actin polymerization tests). As a reagent, recombinant WIPF1 advances both basic cell biology and translational studies in immunology, oncology, and neurology.
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