| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN18 |
| Uniprot No | Q99952 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-460aa |
| 氨基酸序列 | MSRSLDSARS FLERLEARGG REGAVLAGEF SDIQACSAAW KADGVCSTVA GSRPENVRKN RYKDVLPYDQ TRVILSLLQE EGHSDYINGN FIRGVDGSLA YIATQGPLPH TLLDFWRLVW EFGVKVILMA CREIENGRKR CERYWAQEQE PLQTGLFCIT LIKEKWLNED IMLRTLKVTF QKESRSVYQL QYMSWPDRGV PSSPDHMLAM VEEARRLQGS GPEPLCVHCS AGCGRTGVLC TVDYVRQLLL TQMIPPDFSL FDVVLKMRKQ RPAAVQTEEQ YRFLYHTVAQ MFCSTLQNAS PHYQNIKENC APLYDDALFL RTPQALLAIP RPPGGVLRSI SVPGSPGHAM ADTYAVVQKR GAPAGAGSGT QTGTGTGTGA RSAEEAPLYS KVTPRAQRPG AHAEDARGTL PGRVPADQSP AGSGAYEDVA GGAQTGGLGF NLRIGRPKGP RDPPAEWTRV |
| 预测分子量 | 50,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTPN18重组蛋白的参考文献及其摘要概括：
1. **文献名称**: "Protein tyrosine phosphatase PTPN18 regulates HER2 signaling and cell migration in breast cancer cells"
**作者**: Wang Y, et al.
**摘要**: 研究通过重组表达PTPN18蛋白，发现其通过去磷酸化HER2受体酪氨酸激酶抑制乳腺癌细胞迁移，揭示了PTPN18作为肿瘤抑制因子的潜在机制。
2. **文献名称**: "Structural and functional characterization of recombinant human PTPN18 phosphatase domain"
**作者**: Zhang L, et al.
**摘要**: 报道了人源PTPN18催化结构域的重组表达与晶体结构解析，证实其底物特异性依赖于保守的催化活性位点，为开发靶向抑制剂提供结构基础。
3. **文献名称**: "PTPN18 interacts with the EGFR signaling pathway to modulate epithelial cell apoptosis"
**作者**: Chen H, et al.
**摘要**: 利用重组PTPN18蛋白进行体外实验，证明其通过负调控EGFR的磷酸化状态参与上皮细胞凋亡，提示其在组织稳态中的调控作用。
注：以上文献信息为示例性内容，实际文献需通过PubMed或学术数据库检索确认。
PTPN18 (Protein Tyrosine Phosphatase Non-Receptor Type 18) is a member of the protein tyrosine phosphatase (PTP) family, which plays critical roles in regulating cellular signaling pathways by dephosphorylating tyrosine residues on target proteins. This enzyme is implicated in modulating receptor tyrosine kinase (RTK) signaling, cell proliferation, differentiation, and apoptosis. The PTPN18 gene encodes a 50 kDa protein containing a catalytic PTP domain and a proline-rich region, suggesting interactions with SH3 domain-containing proteins. Dysregulation of PTPN18 has been linked to cancer, where it may act as a tumor suppressor or promoter depending on context, particularly in breast and gastric cancers.
Recombinant PTPN18 protein is engineered using expression systems like *E. coli* or mammalian cells to produce a purified, functional form for research. This tool enables studies on its substrate specificity, enzymatic activity, and interactions with signaling molecules (e.g., EGFR, ErbB2). Researchers use it to explore mechanisms underlying PTPN18's role in RTK downregulation, endocytic trafficking, or DNA damage responses. Its phosphorylation-dependent regulation and involvement in pathways like MAPK/ERK or PI3K/AKT are of interest in oncology and drug discovery. Mutational analyses with recombinant protein help identify residues critical for catalysis or binding. Additionally, PTPN18's potential as a therapeutic target or biomarker in diseases driven by tyrosine kinase hyperactivity continues to be investigated.
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