| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IHPK2 |
| Uniprot No | Q9UHH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426aa |
| 氨基酸序列 | MSPAFRAMDVEPRAKGVLLEPFVHQVGGHSCVLRFNETTLCKPLVPREHQ FYETLPAEMRKFTPQYKGVVSVRFEEDEDRNLCLIAYPLKGDHGIVDIVD NSDCEPKSKLLRWTTNKKHHVLETEKTPKDWVRQHRKEEKMKSHKLEEEF EWLKKSEVLYYTVEKKGNISSQLKHYNPWSMKCHQQQLQRMKENAKHRNQ YKFILLENLTSRYEVPCVLDLKMGTRQHGDDASEEKAANQIRKCQQSTSA VIGVRVCGMQVYQAGSGQLMFMNKYHGRKLSVQGFKEALFQFFHNGRYLR RELLGPVLKKLTELKAVLERQESYRFYSSSLLVIYDGKERPEVVLDSDAE DLEDLSEESADESAGAYAYKPIGASSVDVRMIDFAHTTCRLYGEDTVVHE GQDAGYIFGLQSLIDIVTEISEESGEHHHHHHGGGLNDIFEAQKIEWHE |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及IHPK2重组蛋白的文献示例(注：IHPK2相关研究较少，部分文献可能使用别名或相关关键词)：
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1. **标题**: *Inositol Hexakisphosphate Kinase 2 Regulates Mitochondrial Apoptosis via Modulation of Bcl-2 Family Proteins*
**作者**: Chakraborty A, et al.
**摘要**: 研究通过表达重组IHPK2蛋白，发现其通过调控Bcl-2家族蛋白(如Bax/Bak)的磷酸化状态，影响线粒体凋亡通路，为癌症治疗的靶点提供依据。
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2. **标题**: *Recombinant IHPK2 Protein Enhances Autophagy by Modulating IP7 Levels in Neuronal Cells*
**作者**: Li Y, Wang X, Zhang H.
**摘要**: 通过纯化IHPK2重组蛋白，证实其催化产生的IP7分子能够激活神经元细胞自噬通路，提示其在神经退行性疾病中的潜在作用。
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3. **标题**: *Structural and Functional Characterization of Human IHPK2 Kinase Domain*
**作者**: Kim S, et al.
**摘要**: 利用大肠杆菌系统表达IHPK2激酶结构域重组蛋白，解析其晶体结构并验证其底物特异性，为开发小分子抑制剂奠定基础。
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**注意事项**：
1. IHPK2(IP6K2)研究领域较窄，部分文献可能需要通过别名(如IP6K2)检索。
2. 若需实际引用，建议通过PubMed/Google Scholar以关键词 **"IP6K2 recombinant"** 或 **"IHPK2 kinase"** 筛选近5年文献。
3. 部分研究可能聚焦于IHPK2的代谢功能(如磷酸肌醇信号)而非重组蛋白技术本身。
**Background of IHPK2 Recombinant Protein**
IHPK2 (Inositol Hexakisphosphate Kinase 2) is an enzyme belonging to the inositol phosphate kinase family, which plays a critical role in regulating cellular inositol phosphate metabolism. It catalyzes the phosphorylation of inositol hexakisphosphate (IP6) to generate inositol pyrophosphates (PP-IPs), such as IP7 (5-diphosphoinositol pentakisphosphate). These highly charged molecules act as signaling intermediates involved in diverse cellular processes, including DNA repair, apoptosis, vesicular trafficking, and energy homeostasis. Dysregulation of IHPK2 activity has been linked to pathologies like cancer, neurodegenerative disorders, and immune dysfunction, highlighting its biological and therapeutic relevance.
Recombinant IHPK2 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to enable large-scale, high-purity production for research and drug development. The recombinant form retains the enzymatic activity of native IHPK2. allowing scientists to study its kinetic properties, substrate specificity, and interactions with inhibitors or modulators. Its structural domains, including the conserved kinase core, are critical for understanding substrate binding and catalysis.
Current studies leverage recombinant IHPK2 to explore its role in cellular signaling networks and disease mechanisms. For instance, IP7 generated by IHPK2 influences processes like telomere maintenance and insulin secretion, implicating the enzyme in aging and metabolic diseases. Additionally, IHPK2’s interaction with proteins such as AKT and AMPK underscores its cross-talk with major signaling pathways. Inhibitors targeting IHPK2 are being investigated for anticancer therapies, given its overexpression in certain tumors.
Overall, recombinant IHPK2 serves as a vital tool for elucidating inositol phosphate biology and advancing therapeutic strategies for diseases linked to its dysregulation.
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