| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLC3 |
| Uniprot No | Q6P597 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MSVQVAAPGSAGLGPERLSPEELVRQTRQVVQGLEALRAEHHGLAGHLAEALAGQGPAAGLEMLEEKQQVVSHSLEAIELGLGEAQVLLALSAHVGALEAEKQRLRSQARRLAQENVWLREELEETQRRLRASEESVAQLEEEKRHLEFLGQLRQYDPPAESQQSESPPRRDSLASLFPSEEEERKGPEAAGAAAAQQGGYEIPARLRTLHNLVIQYAGQGRYEVAVPLCRQALEDLERSSGHCHPDVATMLNILALVYRDQNKYKEATDLLHDALQIREQTLGPEHPAVAATLNNLAVLYGKRGRYREAEPLCQRALEIREKVLGADHPDVAKQLNNLALLCQNQGKFEDVERHYARALSIYEALGGPHDPNVAKTKNNLASAYLKQNKYQQAEELYKEILHKEDLPAPLGAPNTGTAGDAEQALRRSSSLSKIRESIRRGSEKLVSRLRGEAAAGAAGMKRAMSLNTLNVDAPRAPGTQFPSWHLDKAPRTLSASTQDLSPH |
| 预测分子量 | 57.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLC3重组蛋白的模拟参考文献示例(非真实文献，供格式参考)：
1. **标题**：*Expression and Purification of Recombinant KLC3 in E. coli for Structural Studies*
**作者**：Smith A, et al.
**摘要**：本研究成功克隆了人类KLC3基因，并在大肠杆菌中实现了重组蛋白的高效表达。通过亲和层析纯化获得了高纯度蛋白，为后续晶体结构解析提供了基础。
2. **标题**：*KLC3 Interaction with Motor Proteins: A Yeast Two-Hybrid Analysis*
**作者**：Lee J, et al.
**摘要**：利用酵母双杂交系统，鉴定了KLC3与驱动蛋白重链(KIF5B)的特异性结合域，揭示了其在细胞内囊泡运输中的潜在调控机制。
3. **标题**：*Functional Characterization of Recombinant KLC3 in Neuronal Cell Transport*
**作者**：Chen X, et al.
**摘要**：通过体外重组KLC3蛋白与荧光标记技术，证明其在神经元轴突运输中调控线粒体定位，敲低实验导致运输障碍。
4. **标题**：*Role of KLC3 Phosphorylation in Cancer Cell Metastasis*
**作者**：Wang Y, et al.
**摘要**：构建了KLC3磷酸化突变体重组蛋白，发现其磷酸化状态通过影响与衔接蛋白的结合，显著调控乳腺癌细胞的迁移能力。
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注：以上文献为模拟内容，实际研究中请通过PubMed/Google Scholar等平台检索真实文献。
KLC3 (kinesin light chain 3) is a member of the kinesin superfamily, which plays a critical role in intracellular transport by mediating the movement of cargoes along microtubules. As a regulatory subunit of the kinesin-1 motor complex, KLC3 interacts with the heavy chain (KHC) to facilitate the binding and transport of specific organelles, vesicles, and protein complexes. It is particularly noted for its involvement in spermatogenesis, where it participates in the structural organization of the sperm flagellum and acrosome formation. Dysregulation of KLC3 has been linked to male infertility and certain cancers, underscoring its biological significance.
Recombinant KLC3 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce purified, functional protein for mechanistic studies. Researchers often clone the human *KLC3* gene into expression vectors, enabling controlled production with affinity tags (e.g., His-tag) for simplified purification. This recombinant approach allows for detailed investigations into KLC3’s structure, its interaction partners (e.g., kinesin heavy chains, cargo adaptors), and post-translational modifications that regulate its activity.
Studies leveraging recombinant KLC3 have advanced understanding of microtubule-dependent transport mechanisms, particularly in gamete development and neuronal trafficking. Its role in diseases, such as tumor metastasis linked to aberrant vesicle transport, has also been explored using in vitro assays and animal models. Additionally, recombinant KLC3 serves as a tool for screening therapeutic compounds targeting kinesin-mediated pathways. Ongoing research aims to elucidate its tissue-specific functions and potential as a biomarker or drug target, highlighting its relevance in both basic and applied biomedical sciences.
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