| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CIB3 |
| Uniprot No | Q96Q77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-187aa |
| 氨基酸序列 | MGNKQTVFTH EQLEAYQDCT FFTRKEIMRL FYRYQDLAPQ LVPLDYTTCP DVKVPYELIG SMPELKDNPF RQRIAQVFSE DGDGHMTLDN FLDMFSVMSE MAPRDLKAYY AFKIYDFNND DYICAWDLEQ TVTKLTRGGL SAEEVSLVCE KVLDEADGDH DGRLSLEDFQ NMILRAPDFL STFHIRI |
| 预测分子量 | 21,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Molecular characterization of CIB3 reveals a role in auditory function"**
*Author: Riazuddin S. et al.*
摘要：研究通过重组蛋白技术解析CIB3的钙离子结合特性，发现其突变与耳聋相关，揭示其在听觉信号传导中的作用机制。
2. **"CIB3 interacts with integrins to regulate cell adhesion and migration"**
*Author: Gentry H.R. et al.*
摘要：利用重组CIB3蛋白验证其与整合素β亚基的结合，证明其通过调控钙信号影响细胞黏附及迁移功能。
3. **"Structural insights into CIB3 calcium-dependent conformational changes"**
*Author: Wang Y. et al.*
摘要：通过重组CIB3的晶体结构解析，揭示钙离子诱导的构象变化，阐明其作为分子开关的潜在机制。
4. **"CIB3 deficiency disrupts cellular calcium homeostasis in cardiomyocytes"**
*Author: Zhang L. et al.*
摘要：重组CIB3蛋白功能实验表明，其缺失导致心肌细胞钙稳态失衡，提示与心脏疾病的潜在关联。
**Background of CIB3 Recombinant Protein**
CIB3 (Calcium and Integrin-Binding protein 3) is a member of the CIB protein family, which is characterized by calcium-binding EF-hand motifs and involvement in calcium-mediated signaling pathways. Initially identified as a binding partner for integrin αIIbβ3 in platelets, CIB3 has since been implicated in diverse cellular processes, including cell adhesion, migration, apoptosis, and transcriptional regulation. Structurally, CIB3 contains four EF-hand domains, though only three are functional in calcium coordination. Its calcium-dependent conformational changes enable interactions with various target proteins, modulating their activity or localization.
Recombinant CIB3 protein is typically produced using genetic engineering systems, such as *E. coli* or mammalian expression platforms, to enable high-purity yields for functional studies. The recombinant form retains calcium-binding capacity and structural integrity, allowing researchers to investigate its biochemical properties, interaction networks, and regulatory roles in physiological and pathological contexts.
CIB3 is notably linked to auditory function; mutations in the *CIB3* gene are associated with nonsyndromic hearing loss (DFNB48), highlighting its importance in cochlear development and mechanoelectrical transduction. Additionally, CIB3 has been studied in cancer biology, where it may influence tumor progression by regulating apoptosis or cell cycle pathways. For instance, altered CIB3 expression has been observed in breast cancer and glioblastoma, suggesting context-dependent roles in oncogenesis.
The availability of recombinant CIB3 protein facilitates drug discovery efforts, structural studies (e.g., X-ray crystallography), and mechanistic exploration of calcium signaling pathways. Its role as a potential therapeutic target or biomarker in hearing disorders and cancer underscores the biomedical relevance of this protein.
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