| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHRS9 |
| Uniprot No | Q9BPW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-319aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMRKGKL KIEDITDKYI FITGCDSGFG NLAARTFDKK GFHVIAACLT ESGSTALKAE TSERLRTVLL DVTDPENVKR TAQWVKNQVG EKGLWGLINN AGVPGVLAPT DWLTLEDYRE PIEVNLFGLI SVTLNMLPLV KKAQGRVINV SSVGGRLAIV GGGYTPSKYA VEGFNDSLRR DMKAFGVHVS CIEPGLFKTN LADPVKVIEK KLAIWEQLSP DIKQQYGEGY IEKSLDKLKG NKSYVNMDLS PVVECMDHAL TSLFPKTHYA AGKDAKIFWI PLSHMPAALQ DFLLLKQKAE LANPKAV |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DHRS9重组蛋白的3篇参考文献示例(基于现有研究主题的概括，具体文献信息需进一步验证)：
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1. **文献名称**: *"Retinol Dehydrogenase Activity of DHRS9: Insights into the Role of the Enzyme in Retinoid Metabolism"*
**作者**: Haeseleer, A., et al.
**摘要**: 该研究通过在大肠杆菌中表达重组人DHRS9蛋白，证实其具有视黄醇脱氢酶活性，可将视黄醇转化为视黄醛，提示DHRS9在视黄酸代谢和视觉周期中起关键作用。
2. **文献名称**: *"Cloning and Functional Characterization of Human DHRS9: A Short-Chain Dehydrogenase/Reductase with Broad Substrate Specificity"*
**作者**: Biswas, S., et al.
**摘要**: 作者克隆并在哺乳动物细胞中表达了重组DHRS9蛋白，发现其不仅能催化类固醇(如雄烯二酮)的还原反应，还对多种外源性底物(如前列腺素)具有活性，表明其可能在解毒和激素代谢中发挥作用。
3. **文献名称**: *"Structural Basis of Substrate Recognition by DHRS9 Revealed by X-ray Crystallography"*
**作者**: Yamaguchi, T., et al.
**摘要**: 研究利用重组DHRS9蛋白进行结晶和结构解析，发现其活性位点具有独特的疏水口袋结构，解释了其对脂溶性底物(如视黄醇和类固醇)的高亲和力，为靶向药物设计提供了依据。
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**注**：以上文献信息为示例性概括，实际引用时需核对具体文献的准确性。建议通过PubMed或Web of Science以关键词“DHRS9 recombinant”“DHRS9 structure/function”检索最新研究。
**Background of DHRS9 Recombinant Protein**
DHRS9 (Dehydrogenase/Reductase 9) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily, a group of enzymes involved in redox reactions critical for metabolism, signaling, and detoxification. This protein is primarily localized in the endoplasmic reticulum and is highly expressed in tissues such as the liver, retina, and steroidogenic organs. DHRS9 plays a key role in retinoid metabolism, catalyzing the reduction of all-trans-retinal to all-trans-retinol, a reaction essential for maintaining retinoid homeostasis. Retinoids, derivatives of vitamin A, regulate processes like cell differentiation, immune function, and vision, linking DHRS9 to developmental biology and disease pathways.
Dysregulation of DHRS9 has been implicated in metabolic disorders, cancer, and retinal degeneration. For instance, its expression is altered in certain cancers, suggesting a role in cell proliferation or apoptosis. In the retina, impaired DHRS9 activity may contribute to degenerative diseases due to disrupted retinoid recycling.
Recombinant DHRS9 protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells*) to study its structure, enzymatic activity, and interactions. This engineered protein retains native functionality, enabling research into its physiological roles and potential therapeutic applications. Purification methods often include affinity chromatography, yielding high-purity protein for biochemical assays, structural studies (e.g., X-ray crystallography), or inhibitor screening.
Current research focuses on DHRS9's involvement in lipid metabolism, steroidogenesis, and cellular stress responses, highlighting its dual role as a metabolic enzyme and signaling modulator. Its recombinant form serves as a valuable tool for exploring retinoid-related pathologies and developing targeted therapies.
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