| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TJAP1 |
| Uniprot No | Q5JTD0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-557aa |
| 氨基酸序列 | TSAAPAKKP YRKAPPEHRE LRLEIPGSRL EQEEPLTDAE RMKLLQEENE ELRRRLASAT RRTEALEREL EIGQDCLELE LGQSREELDK FKDKFRRLQN SYTASQRTNQ ELEDKLHTLA SLSHSWIFAI KKAEMDRKTL DWEIVELTNK LLDAKNTINK LEELNERYRL DCNLAVQLLK CNKSHFRNHK FADLPCELQD MVRKHLHSGQ EAASPGPAPS LAPGAVVPTS VIARVLEKPE SLLLNSAQSG SAGRPLAEDV FVHVDMSEGV PGDPASPPAP GSPTPQPNGE CHSLGTARGS PEEELPLPAF EKLNPYPTPS PPHPLYPGRR VIEFSEDKVR IPRNSPLPNC TYATRQAISL SLVEEGSERA RPSPVPSTPA SAQASPHHQP SPAPLTLSAP ASSASSEEDL LVSWQRAFVD RTPPPAAVAQ RTAFGRDALP ELQRHFAHSP ADRDEVVQAP SARPEESELL LPTEPDSGFP REEEELNLPI SPEEERQSLL PINRGTEEGP GTSHTEGRAW PLPSSSRPQR SPKRMGVHHL HRKDSLTQAQ EQGNLLN |
| 预测分子量 | 61,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TJAP1重组蛋白的3篇参考文献示例(注：部分内容基于领域内常见研究方向的模拟概括，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Cloning, Expression, and Purification of Human TJAP1 Recombinant Protein for Functional Studies"*
**作者**：Zhang L, et al.
**摘要**：该研究成功构建了人源TJAP1基因的重组表达载体，通过大肠杆菌系统实现了蛋白的可溶性表达，并利用亲和层析技术纯化获得高纯度TJAP1重组蛋白。实验验证了该蛋白与紧密连接蛋白ZO-1的体外相互作用，为后续功能研究奠定基础。
2. **文献名称**：*"Structural Insights into TJAP1 Role in Epithelial Barrier Regulation via Recombinant Protein Crystallography"*
**作者**：Wang Y, et al.
**摘要**：研究团队利用昆虫细胞表达系统制备了TJAP1重组蛋白，并通过X射线晶体学解析其三维结构。结果显示，TJAP1的特定结构域可能通过介导蛋白-蛋白相互作用参与上皮细胞屏障稳定性的调控。
3. **文献名称**：*"TJAP1 Recombinant Protein Attenuates Inflammatory Signaling in Colonic Epithelial Cells"*
**作者**：Kimura H, et al.
**摘要**：通过哺乳动物细胞表达系统生产功能性TJAP1重组蛋白，并发现其过表达可显著抑制促炎因子(如NF-κB通路)的激活，提示TJAP1可能在肠道炎症性疾病中具有保护作用。
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**注意事项**：
- 以上文献为模拟示例，实际引用时需以真实发表的论文为准。
- 建议通过PubMed、Google Scholar等平台检索关键词(如“TJAP1 recombinant protein”“TJAP1 expression”)获取最新文献。
- 部分研究可能以“TJAP1”的别名(如“PARD3B”)或相关通路(如“tight junction proteins”)为标题，需扩展检索策略。
TJAP1 (Tight Junction-Associated Protein 1), also known as Cingulin-like protein 1. is a cytoplasmic component of tight junctions—specialized cell-cell adhesion structures critical for maintaining epithelial and endothelial barrier function. Initially identified through homology to cingulin, TJAP1 interacts with scaffolding proteins like ZO-1 and participates in organizing the apical junctional complex. It plays roles in regulating cell polarity, proliferation, and signal transduction pathways, including the Hippo and Wnt/β-catenin cascades, which are implicated in tissue homeostasis and cancer progression.
Recombinant TJAP1 protein is engineered for in vitro studies to dissect its molecular interactions and biological roles. Produced using expression systems like *E. coli* or mammalian cells, the protein is often tagged (e.g., His, GST) for purification and detection. Researchers utilize it to investigate TJAP1’s binding partners, its influence on cytoskeletal dynamics, and its involvement in diseases such as epithelial cancers, inflammatory bowel disease, or neurological disorders linked to blood-brain barrier dysfunction. Notably, TJAP1 downregulation has been associated with epithelial-mesenchymal transition (EMT), suggesting its relevance in metastasis.
Studies using recombinant TJAP1 also explore its potential as a therapeutic target or diagnostic marker. For example, altered TJAP1 expression correlates with tumor aggressiveness in certain carcinomas. Additionally, structural analyses of recombinant protein fragments help map functional domains, aiding drug discovery efforts. Despite progress, TJAP1’s full mechanistic spectrum remains under investigation, highlighting the importance of recombinant tools in advancing our understanding of tight junction biology and related pathologies.
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