| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAPK2 |
| Uniprot No | Q9UIK4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MFQASMRSPN MEPFKQQKVE DFYDIGEELG SGQFAIVKKC REKSTGLEYA AKFIKKRQSR ASRRGVSREE IEREVSILRQ VLHHNVITLH DVYENRTDVV LILELVSGGE LFDFLAQKES LSEEEATSFI KQILDGVNYL HTKKIAHFDL KPENIMLLDK NIPIPHIKLI DFGLAHEIED GVEFKNIFGT PEFVAPEIVN YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANIT AVSYDFDEEF FSQTSELAKD FIRKLLVKET RKRLTIQEAL RHPWITPVDN QQAMVRRESV VNLENFRKQY VRRRWKLSFS IVSLCNHLTR SLMKKVHLRP DEDLRNCESD TEEDIARRKA LHPRRRSSTS |
| 预测分子量 | 42,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DAPK2重组蛋白的3篇文献示例及摘要概括：
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1. **文献名称**：**"Recombinant DAPK2 induces autophagy by phosphorylating Beclin-1 and modulating the PI3K/Akt/mTOR pathway"**
**作者**：Li et al.
**摘要概括**：该研究通过在大肠杆菌中表达并纯化重组DAPK2蛋白，发现其通过磷酸化自噬关键蛋白Beclin-1.调控PI3K/Akt/mTOR通路，从而诱导肿瘤细胞自噬。实验表明，DAPK2的激酶活性对自噬的启动至关重要。
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2. **文献名称**：**"Structural and functional characterization of the death domain in recombinant DAPK2"**
**作者**：Garcia-Ruiz et al.
**摘要概括**：作者利用X射线晶体学解析了重组DAPK2蛋白的死亡域结构，揭示了其与促凋亡蛋白相互作用的分子机制。功能实验显示，该结构域对DAPK2介导的细胞凋亡具有决定性作用，并可能成为抗癌药物设计的靶点。
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3. **文献名称**：**"DAPK2 recombinant protein suppresses chronic myeloid leukemia cell proliferation via ERK signaling inhibition"**
**作者**：Wang et al.
**摘要概括**：研究通过昆虫表达系统制备了高活性重组DAPK2蛋白，并在慢性粒细胞白血病模型中验证其功能。结果显示，DAPK2通过抑制ERK信号通路，显著降低白血病细胞的增殖能力，提示其潜在治疗价值。
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这些文献涵盖了DAPK2重组蛋白在自噬调控、结构解析及疾病治疗中的应用，可为相关研究提供方向参考。如需具体文章，建议通过PubMed或Web of Science以关键词“DAPK2 recombinant”进一步检索。
DAPK2 (Death-associated protein kinase 2) is a calcium/calmodulin-regulated serine/threonine kinase belonging to the DAPK family, which plays critical roles in regulating cell death, autophagy, and immune responses. It shares structural homology with other family members, including a conserved kinase domain, a calmodulin-binding regulatory segment, and a cytoskeletal-binding region. Unlike DAPK1. its ubiquitously expressed counterpart, DAPK2 exhibits tissue-specific expression patterns, with higher levels observed in hematopoietic cells, lymphoid tissues, and certain epithelial cells. Its activity is tightly regulated by autophosphorylation and calcium signaling, which modulate its pro-apoptotic functions.
Recombinant DAPK2 proteins are engineered to study its biochemical properties, substrate interactions, and therapeutic potential. Produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells), these proteins retain catalytic activity and structural integrity for in vitro assays. Researchers utilize purified DAPK2 to investigate its role in signaling pathways, particularly its ability to phosphorylate substrates involved in cytoskeletal reorganization or apoptosis initiation. Studies have linked DAPK2 dysregulation to diseases such as leukemia, autoimmune disorders, and neurodegenerative conditions, making it a target for drug discovery. For instance, small-molecule inhibitors targeting its kinase domain are explored for cancer therapy.
Current challenges include elucidating context-dependent signaling mechanisms and resolving structural details of its regulatory domains. Recombinant DAPK2 tools continue to advance our understanding of cell death regulation and its translational applications in precision medicine.
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