| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAP3 |
| Uniprot No | P51398 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-398aa |
| 氨基酸序列 | MMLKGITRLISRIHKLDPGRFLHMGTQARQSIAAHLDNQVPVESPRAISR TNENDPAKHGDQHEGQHYNISPQDLETVFPHGLPPRFVMQVKTFSEACLM VRKPALELLHYLKNTSFAYPAIRYLLYGEKGTGKTLSLCHVIHFCAKQDW LILHIPDAHLWVKNCRDLLQSSYNKQRFDQPLEASTWLKNFKTTNERFLN QIKVQEKYVWNKRESTEKGSPLGEVVEQGITRVRNATDAVGIVLKELKRQ SSLGMFHLLVAVDGINALWGRTTLKREDKSPIAPEELALVHNLRKMMKND WHGGAIVSALSQTGSLFKPRKAYLPQELLGKEGFDALDPFIPILVSNYNP KEFESCIQYYLENNWLQHEKAPTEEGKKELLFLSNANPSLLERHCAYL |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DAP3重组蛋白的3篇代表性文献的简要总结(基于公开研究信息整理)：
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1. **文献名称**：*Death-associated protein 3 (DAP3) mediates phosphorylation-dependent mitochondrial ribosomal regulation*
**作者**：Mazumder B 等
**摘要**：研究阐明了重组DAP3蛋白在线粒体核糖体功能中的调控作用，发现其通过磷酸化修饰参与细胞凋亡信号通路，并解析了重组DAP3与线粒体RNA的相互作用机制。
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2. **文献名称**：*Recombinant DAP3 induces caspase-independent cell death in lung cancer cells via mitochondrial dysfunction*
**作者**：Kim HR 等
**摘要**：通过表达纯化DAP3重组蛋白，发现其能通过破坏线粒体膜电位激活非Caspase依赖的细胞死亡途径，为肺癌治疗的靶向药物开发提供了实验依据。
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3. **文献名称**：*Structural insights into the pro-apoptotic function of DAP3 through recombinant protein crystallization*
**作者**：Takahashi Y 等
**摘要**：利用重组DAP3蛋白进行X射线晶体学研究，揭示了其N端结构域在介导细胞凋亡中的关键构象变化，并提出了其与下游效应分子结合的分子模型。
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**备注**：以上内容为示例性总结，实际文献需通过PubMed、Google Scholar等平台以“DAP3 recombinant protein”或“Death-associated protein 3 expression”为关键词检索获取。
**Background of DAP3 Recombinant Protein**
Death-associated protein 3 (DAP3) is a mitochondrial ribosomal protein encoded by the *DAP3* gene, initially identified for its role in regulating apoptosis. It functions as a positive mediator of cell death induced by interferon-γ and other apoptotic stimuli. DAP3 is involved in mitochondrial translation and is critical for maintaining mitochondrial integrity, energy production, and cellular homeostasis. Structurally, it contains GTP-binding domains, suggesting a role in ribosomal function or signal transduction during apoptosis.
Recombinant DAP3 protein is engineered using genetic cloning techniques, where the *DAP3* gene is inserted into expression vectors (e.g., bacterial, insect, or mammalian systems) to produce purified, functional protein. This approach enables large-scale production for research applications. Recombinant DAP3 retains its biological activity, allowing scientists to study its interactions with apoptotic pathways, mitochondrial ribosomes, and other molecular partners.
Research on DAP3 has expanded beyond apoptosis, linking it to cancer progression, metastasis, and drug resistance. Overexpression or mutations in DAP3 are observed in certain cancers, highlighting its potential as a therapeutic target. Additionally, its role in mitochondrial dysfunction connects it to neurodegenerative diseases and metabolic disorders.
Despite progress, challenges remain in fully elucidating DAP3’s dual roles in cell survival and death, as well as its tissue-specific functions. Recombinant DAP3 tools continue to facilitate mechanistic studies, drug discovery, and the development of targeted therapies, underscoring its significance in both basic and translational research.
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