| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK6 |
| Uniprot No | Q16659 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-721aa |
| 氨基酸序列 | MAEKFESLMN IHGFDLGSRY MDLKPLGCGG NGLVFSAVDN DCDKRVAIKK IVLTDPQSVK HALREIKIIR RLDHDNIVKV FEILGPSGSQ LTDDVGSLTE LNSVYIVQEY METDLANVLE QGPLLEEHAR LFMYQLLRGL KYIHSANVLH RDLKPANLFI NTEDLVLKIG DFGLARIMDP HYSHKGHLSE GLVTKWYRSP RLLLSPNNYT KAIDMWAAGC IFAEMLTGKT LFAGAHELEQ MQLILESIPV VHEEDRQELL SVIPVYIRND MTEPHKPLTQ LLPGISREAL DFLEQILTFS PMDRLTAEEA LSHPYMSIYS FPMDEPISSH PFHIEDEVDD ILLMDETHSH IYNWERYHDC QFSEHDWPVH NNFDIDEVQL DPRALSDVTD EEEVQVDPRK YLDGDREKYL EDPAFDTNYS TEPCWQYSDH HENKYCDLEC SHTCNYKTRS SSYLDNLVWR ESEVNHYYEP KLIIDLSNWK EQSKEKSDKK GKSKCERNGL VKAQIALEEA SQQLAGKERE KNQGFDFDSF IAGTIQLSSQ HEPTDVVDKL NDLNSSVSQL ELKSLISKSV SQEKQEKGMA NLAQLEALYQ SSWDSQFVSG GEDCFFINQF CEVRKDEQVE KENTYTSYLD KFFSRKEDTE MLETEPVEDG KLGERGHEEG FLNNSGEFLF NKQLESIGIP QFHSPVGSPL KSIQATLTPS AMKSSPQIPH QTYSSILKHL N |
| 预测分子量 | 135 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAPK6重组蛋白的模拟参考文献示例(请注意，以下内容为示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Efficient Expression and Purification of Active Recombinant MAPK6 in Insect Cells"
**作者**: Chen L, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统成功表达并纯化了高活性MAPK6重组蛋白，通过体外激酶实验验证其磷酸化功能，为MAPK6的酶学特性研究提供工具。
2. **文献名称**: "Structural Characterization of MAPK6 Reveals Unique Activation Loop Dynamics"
**作者**: Rodriguez S, et al.
**摘要**: 通过X射线晶体学解析MAPK6重组蛋白的三维结构，发现其激活环的独特构象变化，揭示了与经典MAPK家族成员不同的调控机制。
3. **文献名称**: "MAPK6 Recombinant Protein Promotes Cancer Cell Invasion via mTORC1 Signaling"
**作者**: Kim H, et al.
**摘要**: 利用重组MAPK6蛋白进行细胞实验，证明其通过磷酸化mTORC1通路关键蛋白增强乳腺癌细胞的迁移和侵袭能力。
4. **文献名称**: "Development of a MAPK6-Specific Inhibitor Using Recombinant Protein-Based Screening"
**作者**: Müller J, et al.
**摘要**: 基于重组MAPK6蛋白的高通量筛选平台，发现一种小分子抑制剂可特异性阻断MAPK6激酶活性，为靶向治疗提供候选分子。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "MAPK6 recombinant protein" 或 "ERK3 recombinant" 获取真实文献。
**Background of MAPK6 Recombinant Protein**
Mitogen-activated protein kinase 6 (MAPK6), also known as extracellular signal-regulated kinase 3 (ERK3), is an atypical member of the MAPK family. Unlike canonical MAPKs (e.g., ERK1/2), MAPK6 lacks the conserved T-E-Y activation motif and instead possesses a unique S-E-G motif in its activation loop. It is encoded by the *MAPK6* gene and plays roles in diverse cellular processes, including cell cycle regulation, stress responses, and inflammatory signaling. MAPK6 is regulated by phosphorylation and interaction with binding partners, such as MAPK-activated protein kinase 5 (MK5), which influences its stability and subcellular localization.
Structurally, MAPK6 contains a conserved kinase domain but has an extended C-terminal region that contributes to its distinct regulatory mechanisms. Its expression is widespread, with higher levels observed in tissues like the brain, heart, and skeletal muscle. Dysregulation of MAPK6 has been implicated in cancer progression, immune disorders, and neurodegenerative diseases, highlighting its potential as a therapeutic target.
Recombinant MAPK6 protein is produced using expression systems like *E. coli* or insect cells, often fused with tags (e.g., His-tag) for purification. This engineered protein retains kinase activity and enables *in vitro* studies to dissect its signaling pathways, substrate specificity, and interactions. Researchers use it to investigate MAPK6's role in diseases, screen inhibitors, or validate drug candidates. Challenges in its production include maintaining post-translational modifications critical for function, which may require eukaryotic expression systems. Overall, MAPK6 recombinant protein serves as a vital tool for unraveling its biological significance and exploring clinical applications.
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