| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXD2 |
| Uniprot No | O60548 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-495aa |
| 氨基酸序列 | MTLGSCCCEI MSSESSPAAL SEADADIDVV GGGSGGGELP ARSGPRAPRD VLPHGHEPPA EEAEADLAED EEESGGCSDG EPRALASRGA AAAAGSPGPG AAAARGAAGP GPGPPSGGAA TRSPLVKPPY SYIALITMAI LQSPKKRLTL SEICEFISGR FPYYREKFPA WQNSIRHNLS LNDCFVKIPR EPGNPGKGNY WTLDPESADM FDNGSFLRRR KRFKRQPLPP PHPHPHPHPE LLLRGGAAAA GDPGAFLPGF AAYGAYGYGY GLALPAYGAP PPGPAPHPHP HPHAFAFAAA AAAAPCQLSV PPGRAAAPPP GPPTASVFAG AGSAPAPAPA SGSGPGPGPA GLPAFLGAEL GCAKAFYAAS LSPPAAGTAA GLPTALLRQG LKTDAGGGAG GGGAGAGQRP SFSIDHIMGH GGGGAAPPGA GEGSPGPPFA AAAGPGGQAQ VLAMLTAPAL APVAGHIRLS HPGDALLSSG SRFASKVAGL SGCHF |
| 预测分子量 | 48,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与FOXD2重组蛋白相关的3篇参考文献及其摘要概括：
1. **文献名称**: "FOXD2 promotes tumor proliferation and metastasis in hepatocellular carcinoma via the EGFR/ERK signaling pathway"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过重组FOXD2蛋白体外实验，发现其能激活EGFR/ERK通路，促进肝癌细胞增殖和迁移，揭示了FOXD2作为癌症治疗靶点的潜力。
2. **文献名称**: "Recombinant FOXD2 protein enhances chondrogenic differentiation of mesenchymal stem cells through TGF-β/Smad3 activation"
**作者**: Li H, et al.
**摘要**: 研究利用大肠杆菌表达系统制备重组FOXD2蛋白，证实其通过调控TGF-β/Smad3信号通路促进间充质干细胞的软骨分化，为软骨修复提供新策略。
3. **文献名称**: "Structural and functional characterization of the FOXD2 DNA-binding domain using recombinant protein expression"
**作者**: Wang X, et al.
**摘要**: 该研究通过昆虫细胞系统表达并纯化FOXD2 DNA结合域重组蛋白，解析其晶体结构，揭示其特异性识别靶基因启动子区域的关键氨基酸残基。
注：以上文献信息为模拟示例，实际引用时建议通过PubMed/Google Scholar检索真实文献(关键词：FOXD2 recombinant protein, FOXD2 purification)。若需具体文献协助，可提供更详细的研究方向。
FOXD2 (Forkhead box D2) is a member of the Forkhead box (FOX) family of transcription factors, characterized by a conserved DNA-binding "forkhead" domain. It plays regulatory roles in embryonic development, cell differentiation, and tissue-specific gene expression. FOXD2 is notably involved in craniofacial development, kidney morphogenesis, and neural crest cell migration. Studies also link it to cancer progression, where it may act as either a tumor suppressor or promoter depending on context, influencing metastasis and epithelial-mesenchymal transition (EMT).
Recombinant FOXD2 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. Its recombinant form typically includes affinity tags (e.g., His-tag) for efficient purification and detection. Researchers utilize this protein to study DNA-protein interactions, transcriptional regulation mechanisms, and signaling pathways in developmental biology and disease models. It also serves as an antigen for antibody production or a control in assays like Western blotting and EMSA.
Current research focuses on FOXD2's dual roles in cancer and its potential as a therapeutic target. Structural studies of the recombinant protein aim to map functional domains and post-translational modifications that modulate its activity. Challenges include understanding context-dependent regulatory networks and reconciling conflicting roles in different cancers. As a tool, recombinant FOXD2 accelerates mechanistic insights into developmental disorders and oncology, bridging molecular biology with translational applications.
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