| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1qB |
| Uniprot No | P02746 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-253aa |
| 氨基酸序列 | MMMKIPWGSIPVLMLLLLLGLIDISQAQLSCTGPPAIPGIPGIPGTPGPD GQPGTPGIKGEKGLPGLAGDHGEFGEKGDPGIPGNPGKVGPKGPMGPKGG PGAPGAPGPKGESGDYKATQKIAFSATRTINVPLRRDQTIRFDHVITNMN NNYEPRSGKFTCKVPGLYYFTYHASSRGNLCVNLMRGRERAQKVVTFCDY AYNTFQVTTGGMVLKLEQGENVFLQATDKNSLLGMEGANSIFSGFLLFPD MEA |
| 预测分子量 | 26,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C1qB重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际需根据真实文献调整)：
1. **《Recombinant expression and functional characterization of human C1qB in complement activation》**
- 作者：Zhang L, et al.
- 摘要：研究成功在大肠杆菌中表达重组人C1qB蛋白，并通过体外实验证实其与C1q复合物其他亚基的结合能力，证明其在补体经典途径激活中的关键作用。
2. **《Structural and functional analysis of C1qB subunit using a mammalian cell expression system》**
- 作者：Wang Y, et al.
- 摘要：利用哺乳动物细胞系统表达重组C1qB蛋白，结合X射线晶体学分析其三维结构，揭示其与免疫球蛋白结合的分子机制，为靶向补体系统的药物设计提供依据。
3. **《C1qB recombinant protein mitigates lupus nephritis in murine models by modulating complement activity》**
- 作者：Kim S, et al.
- 摘要：通过在小鼠狼疮性肾炎模型中注射重组C1qB蛋白，发现其能抑制补体过度激活，减轻肾脏炎症损伤，提示其作为自身免疫病治疗分子的潜力。
4. **《High-yield purification and biochemical characterization of C1qB for antibody-antigen interaction studies》**
- 作者：Garcia R, et al.
- 摘要：开发了一种高效纯化重组C1qB蛋白的方法，并验证其在体外结合抗原-抗体复合物的能力，强调了其在免疫诊断工具开发中的应用价值。
(注：以上文献为示例，实际引用需检索PubMed、Web of Science等数据库获取真实研究。)
C1qB recombinant protein is a biologically active molecule derived from the C1qB subunit, a critical component of the C1 complex within the complement system. The complement system, a key part of innate immunity, mediates pathogen clearance, immune complex removal, and tissue homeostasis. The C1 complex, composed of C1q, C1r, and C1s, initiates the classical complement pathway upon binding to antigens. C1q, the recognition subcomponent, is a hexameric protein formed by heterotrimers of three chains: C1qA, C1qB, and C1qC. The C1qB chain contributes to the collagen-like region and globular head domains, essential for structural stability and ligand interactions.
Recombinant C1qB is produced using expression systems (e.g., bacterial, mammalian) to enable precise study of its role in immune regulation. Researchers employ it to dissect C1q assembly, ligand-binding mechanisms, and its involvement in diseases like lupus, Alzheimer’s, and atherosclerosis, where aberrant complement activation is implicated. It also aids in developing therapeutic agents targeting complement dysregulation.
The recombinant form offers advantages over native proteins, including higher purity, scalability, and reduced batch variability. It is widely used in ELISA, Western blotting, and functional assays to probe complement activation pathways. Additionally, it serves as a tool for screening inhibitors of C1q-driven inflammation, supporting drug discovery efforts.
Understanding C1qB’s molecular interactions and signaling pathways through recombinant protein studies enhances insights into immune pathologies and potential interventions, bridging basic research and clinical applications.
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