| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHRS4 |
| Uniprot No | Q9BTZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-278aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMHKAGLLGLCARAWNSVRMASSGMTR RDPLANKVALVTASTDGIGFAIARRLAQDGAHVVVSSRKQQNVDQAVATL QGEGLSVTGTVCHVGKAEDRERLVATAVKLHGGIDILVSNAAVNPFFGSI MDVTEEVWDKTLDINVKAPALMTKAVVPEMMHKAGLLGLCARAWNSVRMA SSGMTRRDPLANKVALVTASTDGIGFAIARRLAQDGAHVVVSSRKQEKRG GGSVVIVSSIAAFSPSPGFSPYNVSKTALLGLTKTLAIELAPRNIRVNCL APGLIKTSFSRMLWMDKEKEESMKETLRIRRLGEPEDCAGIVSFLCSEDA SYITGETVVVGGGTPSRL |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DHRS4重组蛋白的3篇代表性文献的简要信息，供参考：
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1. **文献名称**：*DHRS4. a novel tumor suppressor, inhibits proliferation and metastasis by targeting cyclin D1 in hepatocellular carcinoma*
**作者**：Li Y, et al.
**摘要**：该研究通过重组DHRS4蛋白在肝癌细胞中的过表达实验，发现其能够抑制细胞增殖和转移。机制研究表明，DHRS4通过下调cyclin D1的表达阻滞细胞周期，并抑制EMT过程。
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2. **文献名称**：*Characterization of recombinant human DHRS4: a short-chain dehydrogenase/reductase with retinol dehydrogenase activity*
**作者**：Wu L, et al.
**摘要**：文章报道了人源DHRS4重组蛋白在大肠杆菌中的高效表达与纯化，并验证其作为短链脱氢酶/还原酶(SDR)的酶活性。实验表明该蛋白具有视黄醇脱氢酶功能，可能参与维生素A代谢调控。
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3. **文献名称**：*Structural insights into DHRS4-mediated lipid metabolism by X-ray crystallography*
**作者**：Zhang X, et al.
**摘要**：研究通过X射线晶体学解析了重组DHRS4蛋白的三维结构，揭示了其底物结合口袋的构象特征，并发现该蛋白可能通过催化脂质代谢产物影响细胞信号通路。
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**备注**：以上文献为示例性内容，实际文献需通过PubMed、Web of Science等数据库检索确认。建议结合具体研究方向补充近年研究(如DHRS4在癌症、代谢疾病中的功能机制)。
**Background of DHRS4 Recombinant Protein**
DHRS4 (Dehydrogenase/Reductase 4), a member of the short-chain dehydrogenase/reductase (SDR) superfamily, is an NAD(P)-dependent enzyme involved in diverse metabolic pathways. It is encoded by the *DHRS4* gene located on human chromosome 14q11.2. This protein plays roles in retinoid metabolism, steroidogenesis, and cellular detoxification by catalyzing redox reactions, particularly the reduction of carbonyl groups to alcohols or oxidation of alcohols to ketones.
Structurally, DHRS4 contains a conserved Rossmann-fold motif for cofactor binding and a catalytic triad (Ser-Tyr-Lys) typical of SDRs. It exists in multiple isoforms due to alternative splicing, with the predominant isoform being a 30 kDa cytoplasmic protein. Studies suggest its involvement in regulating retinoic acid synthesis by converting retinaldehyde to retinol, linking it to cell differentiation, apoptosis, and embryonic development.
Recombinant DHRS4 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional and structural studies. Its recombinant form enables detailed analysis of substrate specificity, enzymatic kinetics, and interactions with metabolic partners. Researchers utilize it to explore its role in diseases, including cancer (e.g., modulating retinoic acid signaling), metabolic disorders, and oxidative stress-related conditions.
Notably, *DHRS4* forms a gene cluster with its paralog *DHRS4L2* in primates, raising questions about functional redundancy or divergence. Challenges in studying endogenous DHRS4. such as low expression levels, make recombinant proteins vital for elucidating its biological significance. Current research focuses on its therapeutic potential, including drug targeting and biomarker discovery, underscoring its relevance in both basic and applied biomedical sciences.
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