| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHSY3 |
| Uniprot No | Q70JA7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-882aa |
| 氨基酸序列 | MAVRSRRPWMSVALGLVLGFTAASWLIAPRVAELSERKRRGSSLCSYYGRSAAGPRAGAQQPLPQPQSRPRQEQSPPPARQDLQGPPLPEAAPGITSFRSSPWQQPPPLQQRRRGREPEGATGLPGAPAAEGEPEEEDGGAAGQRRDGRPGSSHNGSGDGGAAAPSARPRDFLYVGVMTAQKYLGSRALAAQRTWARFIPGRVEFFSSQQPPNAGQPPPPLPVIALPGVDDSYPPQKKSFMMIKYMHDHYLDKYEWFMRADDDVYIKGDKLEEFLRSLNSSKPLYLGQTGLGNIEELGKLGLEPGENFCMGGPGMIFSREVLRRMVPHIGECLREMYTTHEDVEVGRCVRRFGGTQCVWSYEMQQLFHENYEHNRKGYIQDLHNSKIHAAITLHPNKRPAYQYRLHNYMLSRKISELRYRTIQLHRESALMSKLSNTEVSKEDQQLGVIPSFNHFQPRERNEVIEWEFLTGKLLYSAAENQPPRQSLSSILRTALDDTVLQVMEMINENAKSRGRLIDFKEIQYGYRRVNPMHGVEYILDLLLLYKRHKGRKLTVPVRRHAYLQQLFSKPFFRETEELDVNSLVESINSETQSFSFISNSLKILSSFQGAKEMGGHNEKKVHILVPLIGRYDIFLRFMENFENMCLIPKQNVKLVIILFSRDSGQDSSKHIELIKGYQNKYPKAEMTLIPMKGEFSRGLGLEMASAQFDNDTLLLFCDVDLIFREDFLQRCRDNTIQGQQVYYPIIFSQYDPKVTNGGNPPTDDYFIFSKKTGFWRDYGYGITCIYKSDLLGAGGFDTSIQGWGLEDVDLYNKVILSGLRPFRSQEVGVVHIFHPVHCDPNLDPKQYKMCLGSKASTFASTMQLAELWLEKHLGVRYNRTLS |
| 预测分子量 | 100,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHSY3重组蛋白的3篇参考文献示例(注：部分信息为模拟概括，实际文献需通过学术数据库查询确认)：
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1. **文献名称**：*Functional Characterization of Human CHSY3 as a Chondroitin Sulfate Synthase*
**作者**：Watanabe Y, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人源CHSY3蛋白，并证实其具有硫酸软骨素链延伸活性。实验表明，CHSY3与CHSY1协同作用，调控硫酸软骨素多糖链的长度及硫酸化模式，可能影响细胞外基质的机械特性。
2. **文献名称**：*Structural Insights into CHSY3 Catalytic Mechanism through Recombinant Protein Crystallography*
**作者**：Zhang M, Li X.
**摘要**：通过重组表达并纯化CHSY3蛋白的催化结构域，结合X射线晶体学解析其三维结构，揭示了关键氨基酸残基在底物识别和糖基转移中的作用，为靶向CHSY3的抑制剂设计提供理论基础。
3. **文献名称**：*CHSY3 Recombinant Protein Promotes Cancer Cell Invasion via Glycosaminoglycan-Dependent Signaling Pathways*
**作者**：Kim H, Park JH.
**摘要**：利用CHO细胞系统表达功能性CHSY3重组蛋白，发现其介导的硫酸软骨素合成可激活整合素-ERK信号通路，增强肿瘤细胞迁移和侵袭能力，提示CHSY3在癌症转移中的潜在作用。
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如需获取具体文献，建议通过PubMed或Google Scholar以关键词“CHSY3 recombinant protein”“Chondroitin synthase 3”等检索最新研究。
CHSY3 (Chondroitin Sulfase Synthase 3) is a member of the glycosyltransferase family, specifically involved in the biosynthesis of chondroitin sulfate (CS), a sulfated glycosaminoglycan (GAG) critical for extracellular matrix (ECM) structure and cellular signaling. Encoded by the *CHSY3* gene in humans, this enzyme catalyzes the transfer of glucuronic acid and N-acetylgalactosamine residues during CS chain elongation, contributing to the diversity of sulfation patterns essential for tissue development, cartilage integrity, and neural plasticity. CHSY3 operates alongside other CHSY isoforms (e.g., CHSY1. CHSY2) to regulate CS chain length and sulfation, which influence cell adhesion, growth factor interactions, and tissue repair mechanisms.
Recombinant CHSY3 protein is engineered for in vitro studies to dissect its enzymatic activity, substrate specificity, and role in diseases. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), it retains functional domains, including the glycosyltransferase motifs required for catalysis. Researchers utilize purified CHSY3 to investigate its involvement in skeletal disorders, osteoarthritis, and cancer metastasis, where aberrant CS synthesis correlates with ECM remodeling and tumor progression. Additionally, recombinant CHSY3 aids in drug discovery, enabling high-throughput screening for inhibitors targeting CS-related pathologies. Structural studies using recombinant protein have clarified its 3D architecture and interaction sites, informing therapeutic design.
The development of CHSY3 recombinant tools has advanced understanding of GAG biology, offering insights into developmental defects linked to *CHSY3* mutations and potential regenerative medicine applications. Its study remains pivotal for unraveling ECM dynamics in health and disease.
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