| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHRNg |
| Uniprot No | P07510 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-517aa |
| 氨基酸序列 | MHGGQGPLLLLLLLAVCLGAQGRNQEERLLADLMQNYDPNLRPAERDSDVVNVSLKLTLTNLISLNEREEALTTNVWIEMQWCDYRLRWDPRDYEGLWVLRVPSTMVWRPDIVLENNVDGVFEVALYCNVLVSPDGCIYWLPPAIFRSACSISVTYFPFDWQNCSLIFQSQTYSTNEIDLQLSQEDGQTIEWIFIDPEAFTENGEWAIQHRPAKMLLDPAAPAQEAGHQKVVFYLLIQRKPLFYVINIIAPCVLISSVAILIHFLPAKAGGQKCTVAINVLLAQTVFLFLVAKKVPETSQAVPLISKYLTFLLVVTILIVVNAVVVLNVSLRSPHTHSMARGVRKVFLRLLPQLLRMHVRPLAPAAVQDTQSRLQNGSSGWSITTGEEVALCLPRSELLFQQWQRQGLVAAALEKLEKGPELGLSQFCGSLKQAAPAIQACVEACNLIACARHQQSHFDNGNEEWFLVGRVLDRVCFLAMLSLFICGTAGIFLMAHYNRVPALPFPGDPRPYLPSPD |
| 预测分子量 | 57,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHRNg重组蛋白的3篇代表性文献示例(注：部分信息基于领域内常见研究方向整理，若需具体文献，建议通过学术数据库进一步检索)：
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1. **文献名称**：Expression and functional characterization of recombinant human nicotinic acetylcholine receptor γ subunit in HEK293 cells
**作者**：Smith J, et al.
**摘要**：本研究利用HEK293细胞系统成功表达了重组人源CHRNg蛋白，并通过共转染其他亚基(如α和β)验证了其在功能性烟碱型乙酰胆碱受体组装中的必要性。电生理实验表明，γ亚基的加入显著影响受体的离子通道特性。
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2. **文献名称**：Purification and structural analysis of CHRNg extracellular domain using Escherichia coli expression system
**作者**：Zhang Y, et al.
**摘要**：作者开发了一种基于大肠杆菌的重组CHRNg胞外域表达和纯化策略，通过亲和层析和尺寸排阻色谱获得高纯度蛋白。X射线晶体学分析揭示了其关键配体结合区域的构象特征，为药物靶点研究提供结构基础。
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3. **文献名称**：Role of the γ subunit in nicotine-induced receptor upregulation: Insights from recombinant protein models
**作者**：Johnson R, et al.
**摘要**：通过体外重组CHRNg与其他受体亚基的共表达，研究发现γ亚基在尼古丁诱导的受体上调机制中起调控作用。该研究为理解尼古丁成瘾的分子机制提供了新视角。
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**备注**：上述文献为示例性质，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“CHRNg recombinant protein”“nicotinic receptor gamma subunit expression”进行精确查找。
**Background of CHRNg Recombinant Protein**
The CHRNg recombinant protein is derived from the human *CHRNg* gene, which encodes the gamma subunit of the nicotinic acetylcholine receptor (nAChR). nAChRs are ligand-gated ion channels critical for synaptic transmission in the nervous system and neuromuscular junctions. These receptors are pentameric, typically composed of combinations of alpha (α), beta (β), gamma (γ), delta (δ), and epsilon (ε) subunits. The gamma subunit (CHRNg) is predominantly expressed during early developmental stages and in fetal muscle, where it contributes to the formation of functional receptor complexes before being replaced by the epsilon subunit in adult neuromuscular junctions.
Recombinant CHRNg is produced using biotechnological methods, such as expression in mammalian or bacterial systems, followed by purification to ensure homogeneity. This engineered protein enables researchers to study the structural and functional roles of the gamma subunit in receptor assembly, ligand binding, and ion channel gating. It is particularly valuable for investigating developmental transitions in nAChR composition and understanding pathologies linked to receptor dysfunction, such as congenital myasthenic syndromes or nicotine addiction.
In research, CHRNg recombinant protein is utilized in electrophysiology, binding assays, and structural studies (e.g., cryo-EM) to dissect receptor mechanisms. It also aids in drug discovery, serving as a target for screening compounds that modulate nAChR activity. By providing a controlled source of the gamma subunit, this tool enhances our ability to explore neuromuscular development, synaptic plasticity, and cholinergic signaling pathways, bridging gaps between molecular biology and therapeutic innovation.
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