| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SND1 |
| Uniprot No | Q7KZF4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-910aa |
| 氨基酸序列 | MASSAQSGGSSGGPAVPTVQRGIIKMVLSGCAIIVRGQPRGGPPPERQINLSNIRAGNLARRAAATQPDAKDTPDEPWAFPAREFLRKKLIGKEVCFTIENKTPQGREYGMIYLGKDTNGENIAESLVAEGLATRREGMRANNPEQNRLSECEEQAKAAKKGMWSEGNGSHTIRDLKYTIENPRHFVDSHHQKPVNAIIEHVRDGSVVRALLLPDYYLVTVMLSGIKCPTFRREADGSETPEPFAAEAKFFTESRLLQRDVQIILESCHNQNILGTILHPNGNITELLLKEGFARCVDWSIAVYTRGAEKLRAAERFAKERRLRIWRDYVAPTANLDQKDKQFVAKVMQVLNADAIVVKLNSGDYKTIHLSSIRPPRLEGENTQDKNKKLRPLYDIPYMFEAREFLRKKLIGKKVNVTVDYIRPASPATETVPAFSERTCATVTIGGINIAEALVSKGLATVIRYRQDDDQRSSHYDELLAAEARAIKNGKGLHSKKEVPIHRVADISGDTQKAKQFLPFLQRAGRSEAVVEYVFSGSRLKLYLPKETCLITFLLAGIECPRGARNLPGLVQEGEPFSEEATLFTKELVLQREVEVEVESMDKAGNFIGWLHIDGANLSVLLVEHALSKVHFTAERSSYYKSLLSAEEAAKQKKEKVWAHYEEQPVEEVMPVLEEKERSASYKPVFVTEITDDLHFYVQDVETGTQLEKLMENMRNDIASHPPVEGSYAPRRGEFCIAKFVDGEWYRARVEKVESPAKIHVFYIDYGNREVLPSTRLGTLSPAFSTRVLPAQATEYAFAFIQVPQDDDARTDAVDSVVRDIQNTQCLLNVEHLSAGCPHVTLQFADSKGDVGLGLVKEGLVMVEVRKEKQFQKVITEYLNAQESAKSARLNLWRYGDFRADDADEFGYSR |
| 预测分子量 | 101,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与SND1重组蛋白相关的文献摘要示例(文献信息为模拟概括，仅供参考)：
1. **《Recombinant SND1蛋白的体外表达及其核酸酶活性分析》**
*作者：Zhang Y, et al.*
摘要：研究通过大肠杆菌系统成功表达并纯化重组SND1蛋白，验证其具有依赖Ca²⁺的核酸酶活性，为后续功能研究提供实验基础。
2. **《Structural insights into the tandem Tudor domains of SND1 in gene regulation》**
*作者：Li X, et al.*
摘要：通过X射线晶体学解析SND1重组蛋白的串联Tudor结构域三维结构，揭示其结合RNA及参与基因沉默复合体(RISC)的分子机制。
3. **《SND1重组蛋白促进肿瘤细胞侵袭的分子机制研究》**
*作者：Wang H, et al.*
摘要：利用体外重组SND1蛋白实验，证明其通过激活NF-κB信号通路增强乳腺癌细胞迁移和侵袭能力，提示其作为癌症治疗靶点的潜力。
4. **《SND1 interacts with Argonaute proteins in RNA-induced silencing complexes》**
*作者：Smith J, et al.*
摘要：通过重组SND1与Argonaute蛋白的体外结合实验，证实SND1作为RISC复合体的关键组分，参与miRNA介导的基因表达调控过程。
(注：以上为基于领域知识的模拟文献，实际引用请以真实出版物为准。)
**Background of SND1 Recombinant Protein**
Staphylococcal nuclease domain-containing protein 1 (SND1), also known as Tudor-SN or p100. is a multifunctional protein implicated in transcriptional regulation, RNA processing, and cellular signaling. Initially identified as a co-activator in Epstein-Barr virus-mediated transcription, SND1 has since been linked to diverse biological processes, including mRNA splicing, RNA interference, and miRNA-mediated gene silencing. Structurally, SND1 contains four tandem staphylococcal nuclease-like domains (SN1–SN4) and a Tudor domain, which facilitate interactions with nucleic acids, proteins, and PIWI-family proteins involved in RNA silencing pathways.
SND1 plays critical roles in both normal physiology and disease. It is essential for maintaining cellular homeostasis, regulating stress responses, and modulating immune signaling pathways, such as NF-κB. However, dysregulation of SND1 is associated with cancer progression, inflammation, and neurodegenerative diseases. Overexpression of SND1 has been observed in multiple cancers (e.g., hepatocellular carcinoma, breast cancer), where it promotes tumor growth, invasion, and chemoresistance by enhancing oncogenic signaling and suppressing apoptosis.
Recombinant SND1 protein is engineered for *in vitro* and *in vivo* studies to dissect its molecular functions and therapeutic potential. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains for binding nucleic acids or partner proteins. Researchers utilize recombinant SND1 to investigate its role in RNA metabolism, protein-protein interactions, and cancer pathways. Additionally, it serves as a tool for screening inhibitors targeting SND1 activity in drug discovery.
The development of SND1 recombinant protein underscores its importance as a biomarker and therapeutic target, driving advances in understanding its dual roles in cellular regulation and disease mechanisms.
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