| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR39 |
| Uniprot No | O43194 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MASPSLPGSDCSQIIDHSHVPEFEVATWIKITLILVYLIIFVMGLLGNSA TIRVTQVLQKKGYLQKEVTDHMVSLACSDILVFLIGMPMEFYSIIWNPLT TSSYTLSCKLHTFLFEACSYATLLHVLTLSFERYIAICHPFRYKAVSGPC QVKLLIGFVWVTSALVALPLLFAMGTEYPLVNVPSHRGLTCNRSSTRHHE QPETSNMSICTNLSSRWTVFQSSIFGAFVVYLVVLLSVAFMCWNMMQVLM KSQKGSLAGGTRPPQLRKSESEESRTARRQTIIFLRLIVVTLAVCWMPNQ IRRIMAAAKPKHDWTRSYFRAYMILLPFSETFFYLSSVINPLLYTVSSQQ FRRVFVQVLCCRLSLQHANHEKRLRVHAHSTTDSARFVQRPLLFASRRQS SARRTEKIFLSTFQSEAEPQSKSQSLSLESLEPNSGAKPANSAAENGFQE HEV |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR39重组蛋白的3篇模拟参考文献示例(基于已有研究方向的合理推测，非真实文献)：
---
1. **标题**: "Recombinant Expression and Functional Characterization of GPR39 in HEK293 Cells"
**作者**: Smith A, et al.
**摘要**: 研究报道了在HEK293细胞中成功表达重组GPR39蛋白，并证实其可被Zn²⁺激活，通过Gq和Gi信号通路调控细胞内cAMP和钙离子水平，为研究GPR39的代谢调控功能提供了工具。
2. **标题**: "Structural Insights into GPR39 Activation by Synthetic Agonists"
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜技术解析了重组GPR39与合成小分子激动剂结合的复合物结构，揭示了受体跨膜结构域的构象变化，为靶向GPR39的药物设计提供了结构基础。
3. **标题**: "Development of a Bioluminescence Resonance Energy Transfer (BRET) Assay for GPR39 Activity Screening"
**作者**: Lee J, et al.
**摘要**: 利用重组GPR39蛋白构建BRET检测体系，筛选并验证了多种候选配体对受体下游β-arrestin招募的影响，发现新型GPR39变构调节剂。
---
**备注**：以上内容为基于领域知识的模拟示例，实际文献需通过PubMed/Google Scholar等平台检索关键词“GPR39 recombinant protein”“GPR39 structure/function”获取。
GPR39 (G Protein-Coupled Receptor 39) is a class A G protein-coupled receptor (GPCR) that has garnered increasing attention for its potential roles in metabolic regulation, cellular signaling, and disease pathophysiology. Initially identified as an orphan receptor due to its unknown endogenous ligands, GPR39 was later found to be activated by zinc ions (Zn²⁺), which act as its primary endogenous agonist. It is widely expressed in tissues such as the gastrointestinal tract, pancreas, brain, and adipose tissue, suggesting diverse physiological functions. Structurally, GPR39 shares homology with ghrelin and motilin receptors but exhibits unique signaling properties, including coupling to Gαq/11 and Gα12/13 proteins, which mediate pathways like phospholipase C (PLC) activation, intracellular calcium mobilization, and Rho kinase signaling.
Recombinant GPR39 protein, typically produced via heterologous expression systems (e.g., mammalian or insect cells), is engineered for research applications to study receptor-ligand interactions, signaling mechanisms, and structural features. Its recombinant form often includes tags (e.g., FLAG, HA, or His-tag) to facilitate purification, localization, and detection in experimental models. Studies using recombinant GPR39 have revealed its involvement in insulin secretion, appetite regulation, and cell survival, with implications for metabolic disorders such as diabetes and obesity. Additionally, GPR39 has been linked to cancer progression and neurological conditions, though its exact mechanisms remain under investigation.
Despite advances, challenges persist in fully elucidating GPR39's ligand selectivity, biased signaling, and tissue-specific roles. Recombinant protein tools remain critical for developing therapeutic agents targeting GPR39. particularly for metabolic and neurodegenerative diseases. Ongoing research aims to resolve its crystal structure and identify synthetic modulators, which could unlock novel treatment strategies.
×
