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| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | INHB |
| Uniprot No | O61643 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-946aa |
| 氨基酸序列 | MRFAFDSNHSQSGAPFKGSRCFFNCQCICCRQGCCVVVVKCCCCFNLNCCNSLGSRKSFPQPAAMRKKVADLEVLRVSRFVAVILVLARWVTAVATLLTSCILLDIFSVPGQSGVADRSQASSRTVHVSVPTTPNETPSSTSETKLKLLYGYTSYDINNDQQVKSNNLCRVLCKSRNRKRQRRRRRRRNHRRRRHRYTKRLHHLMQDNMSGFEQRLNFSDAKCQSLETNYGTNYDLVQGGKLFSQSERSLLVSPLREIEAPWPAIHGSMRNCSKIKRNRANLIWLLIGLVWFEVKLINCNGISSSNYYASNLESHKGCTLCHESGKPNIYTDKDNPHTDYNIYNKYHSNNNFNKKTNQPHNNIAPSDEVRLESIKRQILTKLGLSHKPNVSHPLPKQFIWETIYRVDGGRMIPNNAFGSSGKNLDQKTIKLRAFASPGSHLFNGRGGRTDQRSERDPSHHKYRSPFDFTFNISKNNVYGKVLRNRSLERIDKKNSFLNGWTENRQLKINSQIASMPIELKSHHNSSPKELKSGAVRKVNGINGTQMNENALKKSTYPIDINHSIDNKTHTGKNGEMSHNDYEYFNDYSVQTHDKNRYHEGRSSIGYQPAIHNIEYENQKGHHESFADDHENIDHEDFFGNTQEIITFAEEGTQYRQYRILEFSAQNRRVPSQKLSIRSAQIHIRIDKPHSLWIEKAKSLPEKHLLNTKRKWGANKPHHRIKIWVFQLSTSINITEKGIDKAIIFRASFQVDPKNLGWQKFDLTDTIREWYGHTSHEKLRLLIDCTGCGGRYSLHLFQTSKLRGNSSDYLSTNPNRPFLVLHTESSRTRRVRRRAVDCGGALNGQCCKESFYVSFKALGWDDWIIAPRGYFANYCRGDCTGSFRTPDTFQTFHAHFIEEYRKMGLMNGMRPCCAPIKFSSMSLIYYGDDGIIKRDLPKMVVDECGCP |
| 预测分子量 | 108,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于INHB(抑制素B)重组蛋白的3篇参考文献示例(内容基于学术文献的常见研究方向，仅供参考)：
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1. **文献名称**：*Recombinant human inhibin B: production, characterization, and biological activity*
**作者**：Schneyer, A.L., Hall, H.A., Lambert-Messerlian, G.
**摘要内容**：该研究描述了利用哺乳动物细胞表达系统(如CHO细胞)生产重组人抑制素B的方法，并通过Western blot和质谱分析验证其纯度与结构。实验表明，重组INHB能够有效抑制FSH(促卵泡激素)的分泌，证实其生物活性与天然蛋白一致。
2. **文献名称**：*Development of a sensitive ELISA for inhibin B using recombinant protein as a standard*
**作者**：Robertson, D.M., Stephenson, T., Pruysers, E.
**摘要内容**：研究团队开发了一种基于重组抑制素B的高灵敏度ELISA检测方法，用于精确量化血清中的INHB水平。通过与天然蛋白对比，验证了重组INHB作为校准品的可靠性，该方法在评估男性精子生成功能和女性卵巢储备中具有临床价值。
3. **文献名称**：*Role of recombinant inhibin B in modulating gonadal steroidogenesis*
**作者**：Hayes, F.J., Pitteloud, N., DeCruz, S.
**摘要内容**：通过体外细胞模型，研究发现重组INHB可直接抑制睾丸支持细胞和卵巢颗粒细胞的类固醇生成(如睾酮和雌二醇)，揭示了其在生殖内分泌调控中的双重作用，为多囊卵巢综合征(PCOS)等疾病的机制研究提供依据。
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**说明**：以上文献为示例性内容，实际引用时需根据具体研究领域核实真实存在的文献。建议通过PubMed、Web of Science等数据库检索关键词“recombinant inhibin B”或“INHB recombinant protein”获取最新研究。
Inhibin beta (INHB) is a subunit of inhibin, a glycoprotein hormone belonging to the transforming growth factor-beta (TGF-β) superfamily. Inhibins are heterodimers composed of an alpha subunit (INHA) and one of two beta subunits (INHB or INHBA), forming inhibin A (α-βA) or inhibin B (α-βB). These hormones play critical roles in regulating follicle-stimulating hormone (FSH) secretion from the pituitary gland, primarily through negative feedback mechanisms. INHB is particularly important in reproductive physiology, influencing gonadal function, folliculogenesis in females, and spermatogenesis in males.
Recombinant INHB protein is produced using genetic engineering techniques, often expressed in mammalian cell systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. This engineered protein retains the biological activity of native inhibin B, enabling researchers to study its functions in vitro and in vivo. Its applications span basic research on reproductive endocrinology, clinical studies of disorders like polycystic ovary syndrome (PCOS) and premature ovarian insufficiency, and potential diagnostic/therapeutic development.
Recent studies highlight INHB's emerging role as a biomarker for ovarian reserve and testicular function. In assisted reproductive technologies, inhibin B levels help assess ovarian response to stimulation. Recombinant INHB also aids in developing assays to detect autoimmune infertility caused by anti-inhibin antibodies. Pharmaceutical interest focuses on its potential for modulating FSH activity in conditions like osteoporosis or hormone-sensitive cancers. The availability of recombinant INHB has significantly advanced understanding of inhibin signaling pathways and their crosstalk with activins, which share beta subunits but exert opposing biological effects.
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